ABSTRACT
The study investigates the antibacterial and antifungal activity of some actinomycetes microorganisms. Twenty actinomycetes were isolated from different soils at various locations in Egypt. Cultural, morphological and physiological characteristic of this isolates indicated that they belong to Streptomyces sp. The isolates have antagonistic activity against different Gram negative and Gram positive multi-drug resistant bacteria and pathogenic fungi. Two isolates namely Streptomyces bobili FS-3 and Streptomyces poonensis FS-4 were further investigated. The potency of antimicrobial activity depend on incubation time, the nutritional factors such as carbon, nitrogen and phosphorus sources and environmental factors such a initial pH, medium capacity, size, type and age of inoculum