ABSTRACT
Objectives: To evaluate and investigate the patients of Beta Thalassemia Major, presenting with anemia, organomegalies, skeletal expansion, and high fetal hemoglobin, for end organ damage and endocrine abnormalities with iron overload
Methods: diagnosed cases of Beta Thalassemia Major were included in this study and the parameters analyzed were: Hemoglobin, MCV, Hematocrit, RBCs, RDW, Reticulocytes, HbF, HbA2, Ferritin, LDH, Creatinine, Blood Glucose Random, LH, FSH, and TSH
Results: Mean value of different parameters were Hemoglobin 5.5 g/dL, MCV 76.1fL, Hematocrit 19.2%, RBCs 2.53x109/L, RDW 33.7fL, Reticulocytes 12.7%, HbF 73.3%, HbA2 2.03%, Ferritin 3212 ng/mL, LDH 2237 U/L, Creatinine 1.3g/dL, Blood Glucose Random 110mg/dL, LH 6.5 U/L, FSH 7.8U/L, and TSH 2.4 microU/mL
Conclusion: All patients having microcytic, hypochromic anemia, low MCV, high RDW, nucleated RBCs in peripheral blood, and reticulocytosis should be subjected to work up for hemolysis such as LDH, DAT, and hemoglobin electrophoresis. Above mentioned findings are usually coupled with skeletal expansion and organomegalies in case of Beta Thalassemia Major. In addition, after the confirmation of Beta Thalassemia Major, iron overload must be assessed to incorporate an effective iron chelating therapy
ABSTRACT
Objective: 1. To identify the fluorescent signal pattens on WBC histograms in flowcytometry based, five parts differential haematology analyzers, in smear positive cases. 2. To make a diagnostic algorithm based on haematological parameters and WBC scattergrams in suspicious cases of P vivax malaria
Methods: seventy smear positive Plasmodium vivax haematological indices on automated 5 part differential hematology analyzers [XE2100 and XE5000] were checked for seminal use as potential diagnostic markers
Results: seventy smear positive Plasmodium vivax malaria cases were selected. Haematological indices revealed that 83.4% had thrombocytopenia. Pseudoeosinophilia was seen in 76.4% cases with 51/68 showing more than 5% gap. Considerable anaemia [Hb <10 g/dl] was exhibited by 32.8% of the patients. Leukopenia was seen only in 11 cases. Monocytosis was seen in 30 patients
Conclusion: CBC run on automated analyzers gives information in the form of an integrated pattern of results. When thrombocytopenia along with raised MPV and POW, anemia, leukopenia, monocytosis, eosinophilia [Pseudoeosinophilia in actual], abnormalities of WBC scatter-grams, monocytosis and altered ROW are read in collaboration lead to strong suspicion of P vivax malaria infestation