Rapid diagnosis of malaria by fluorescent microscopy with light micrsoscope and interference filter

The present study is planned to compare acridine orange [A.O] staining with Giemsa staining by using light microscopy with IF and also with fluorescent microscopy for detection of parasites in peripheral blood of patients suffering from clinically suspected cases of malaria. 200 patients with fever and shivering were included. General investigations like Hb, TLC and platelets were done by sysmex K-1000. Thin and thick blood films were made and stained according to protocol given i.e.by giemsa and AO stains and slides were examined by different microscopes i.e. light microscope, light positive parasitemia and 30 [15%] subjects were negative for malaria parasites. Fib, TLC and platelets were reduced when comparing with MP negative cases. IFS microscope with acridine orange staining showed early detection of malaria parasites by counting fewer fields as compared to light microscopy with Giemsa stains. Time consumed for detection of parasites was also significantly reduced in IFS microscope by using AO stains

Portal triad changes in liver associated with rapeseed oil diet supplemented with atherogenic element: an experimental study

This experimental study was designed to see the possible effects of rapeseed oil on liver morphology. For this purpose, 60 albino rats of eight weeks age were selected and divided into five groups of twelve animals each with equal number of males and females. Group I, normal control, was fed on synthetic diet, Group II was on low rapeseed oil diet only and group III was on low rapeseed + hypercholesterolemic diet. Group IV was on high rapeseed oil diet only and Group V was on high rapeseed oil + hypercholesterolemic diet for the next 24 weeks. Histological examination was done on H and E, reticulin, trichrome and oil red O stains. Liver revealed mild to severe type of Portal triad fibrosis in groups II, III, IV and V. These findings were statistically significant when compared with control Group I. While bile duct proliferation [BDP] was mild in Group II and IV. Hence it is concluded that Rapeseed oil proved to be beneficial diet. It causes mild hepatic injury in low and high concentration. Hypercholesterolemic diet causes moderate to severe morphological changes in liver

Role of ultrasound in rotator cuff injury

To determine the role of ultrasound [US] in Rotator cuff pathologies and to correlate the accuracy of Ultrasonography [USG] findings in rotator cuff pathologies with b Magnetic Resonance Imaging. It was a Descriptive study. Study was conducted in Department of Radiology, PNS Shifa Karachi, where Grey Scale as well as Color Doppler USG and Fluoroscopy was available. However the facilities of MRI were utilized from other Radiology departments in Karachi; namely, National Medical Centre [NMC], Advanced Radiology Clinic [ARC] and Agha Khan University Hospital [AKUH] on referral basis. The study involved 50 patients of both sexes between 15-60 years of age who presented with shoulder pain and stiffness, in out-patient departments or admitted in surgical/orthopedic wards. They were referred to the Radiology department PNS Shifa for X-Rays and US shoulder. All of them were followed by Magnetic Resonance Imaging. Patients were 32 males and 18 females of more than 15 years of age. Rotator cuff tears were suggested on US by finding thinning or absence of cuff, by hyper or hypo echoic areas in cuff and by criteria of the abnormal cuff-bursa interface. Out of 50 patients 35 patients were diagnosed on US, as having rotator cuff tears while 15 patients were reported normal. 21 out of 35 patients were having full thickness tear while 14 had partial thickness tears. When MRI was conducted in these patients, it showed 39 rotator cuff tears out of 50 patients while 11 patient

ere diagnosed as normal. 26 out of 39 patients were having full thickness tears while 13 had partial thickness tears. Ultrasound had high sensitivity, specificity, positive predictive values and accuracy. We conclude that MRI and US are both sensitive in detection of abnormalities of the rotator cuff. In our set up; Ultrasound is an accurate, non invasive and economic mode of investigation for diagnosing rotator cuff pathologies