ABSTRACT
Background: Previous studies from animal models have shown that pre-synapticNMDA receptors (preNMDARs) are present in the cortex, but the role of inhibition mediated bypreNMDARs during epileptogenesis remains unclear. In this study, we wanted to observe thechanges in GABAergic inhibition through preNMDARs in sensory-motor and visual corticalpyramidal neurons after pilocarpine-induced status epilepticus.Methods: Using a pilocarpine-induced epileptic mouse model, sensory-motor and visualcortical slices were prepared, and the whole-cell patch clamp technique was used to recordspontaneous inhibitory post-synaptic currents (sIPSCs).Results: The primary finding was that the mean amplitude of sIPSC from the sensorymotorcortex increased significantly in epileptic mice when the recording pipette contained MK-801 compared to control mice, whereas the mean sIPSC frequency was not significantly different,indicating that post-synaptic mechanisms are involved. However, there was no significant presynapticinhibition through preNMDARs in the acute brain slices from pilocarpine-inducedepileptic mice.Conclusion: In the acute case of epilepsy, a compensatory mechanism of post-synapticinhibition, possibly from ambient GABA, was observed through changes in the amplitude withoutsignificant changes in the frequency of sIPSC compared to control mice. The role of preNMDARmediatedinhibition in epileptogenesis during the chronic condition or in the juvenile stagewarrants further investigation.
ABSTRACT
Community engagement efforts in brains and neurosciences projects involving higher education institutions are currently sporadic in Malaysia and likely to contribute the apparent lack of neuroscience awareness in the society. In this paper, we highlight ‘The Brain Apprentice’ project as a knowledge transfer effort to raise neuroscience awareness using school-centred neuroscience clubs. These groups promote the appreciation of neuroscience beyond conventional classroom approaches and the training of neuroscience graduate interns as student facilitators in the teaching and learning of neuroscience. The Brain Apprentice was delivered through the establishment of two school-based neuroscience clubs, Sekolah Kebangsaan Kubang Kerian 3 (primary level) and Sekolah Menengah Sains Tengku Muhammad Faris Petra (secondary level). The teaching and learning of neuroscience was delivered through practical sessions and competitions. Questionnaires were collected from the students based on the following four domains: general satisfaction, impact of knowledge transfer, satisfaction with graduate interns, and knowledge and practical relevance of neuroscience. The National Brain Bee championship has resulted in the first Malaysia representative competing at the International Brain Bee 2012. Students, who had participated as neuroscience club members were exposed to the basic principles of neuroscience, which boosted their interest in science and neuroscience. The graduate interns had also been provided with opportunities to hone in their soft skills and be involved in community-engagement efforts. This project offered a suitable model of community-engagement in raising awareness about and the profile of neuroscience both in terms of knowledge exposure and from the perspective of career options in the field.