ABSTRACT
Allopathic system of medication is the most popular and preferable approach of the treatment in the current scenarios, but in spite of the wide use of allopathic medicine, the trend of use of herbal medicines are also valuable contributor with the current medicinal therapies in the health care professions. In this connection, an eastern use of Morus nigra L. fruit was formulated into solid dosage from named as Lozenges. The lozenges consisted of Morus nigra L. aqueous extract, which dissolves in the mouth providing localized effect against sore throat and cough. A full-scale batch of lozenges trial was manufactured to ensure uniform process and analytical parameters. The un-packed lozenges were evaluated for qualitative specifications like, average weight which was about 2.250-2.750 [2.5 g +/- 10%] and uniformity of weight was 18/20-NMT +/- 10% 2/20. The results were found within the official limits. The appearance of the lozenges was checked organoleptically while dimensions were evaluated using calibrated vernier caliper. Packed and finished lozenges dosage form were evaluated for microbiological test like, aerobic bacteria, fungi, P.aeruginosa, E. coli, Staphylococcus aureus, Salmonella and other enteric bacteria. Thin Layer Chromatography [TLC] confirmed the presence of flavonoids as anthocyanins at 254 nm. The quantity of flavonoids as anthocyanin [cyaniding-3-glucoside] was determined as not less than [NLT] 0.80 mg/Loz. Caffeic acid was also estimated quantitatively using High Performance Thin Layer Chromatography [HPTLC] and was found NLT 0.002 mg/loz