ABSTRACT
Objective: To determine the effect of Vildagliptin in non-alcoholic, fatty liver disease patients with dyslipidemia
Methods: A randomized placebo controlled trial was conducted at outpatient clinic of Medical Unit-I of Sheikh Zayed Medical College/Hospital, Rahim Yar Khan, in which fifty eight patients of NAFLD with dyslipidemia were divided in to two, case and control groups. The case group was given tablet Vildagliptin 50mg twice a day for twelve weeks and control group was given placebo in same way. Body weight, body mass index [BMI], lipid profile, liver enzymes and ultrasound finding of fatty liver were assayed before and after treatment
Results: After 12 weeks treatment of vildagliptin there was significant improvement in following parameters. Body weight and BMI decreased significantly from 88 +/- 11 to79 +/- 12 kg [p0.036] and 30 +/- 4 to 27 +/- 5 kg/m2 [p 0.005] respectively. Notable reduction in the value of TC, TG and LDL-C [TC: 252 +/- 24 to 220 +/- 20mg/dl [p 0.031]; TG: 190 +/- 24 to115 +/- 22 mg/dl [p 0.005]; LDL-C 160 +/- 15 to 145 +/- 13mg/dl [p 0.004]. HDL-C level increased significantly from 29 +/- 5to45 +/- 4 mg/dl [p 0.001].There was remarkable reduction in aminotransferases level [ALT: 78 +/- 17 to 48 +/- 14IU/L [p 0.036]. AST: 63.3 +/- 13 to41 +/- 11IU/L [p 0.002]. There was overall 65.5% improvement in fatty liver grading on ultrasound with vildagliptin while non significant effects were seen in placebo group in all of the above parameters
Conclusion: Vildagliptin exhibited beneficial effects in non-alcoholic fatty liver disease, Nondiabetic patients with dyslipidemia
ABSTRACT
Background: Different methods of heparin extraction are being used
Objective: To compare heparin extraction from bovine pancreas by using three different methods including method of Charles and Scott, Max and Volpi
Methodology: Design: Comparative Study. Setting: The University of Lahore with three month's duration starting from 1[st] July 2013. Fresh twenty samples of bovine pancreas samples were collected from the slaughterhouse of Lahore Pakistan and immediately placed in the ice buckets. The isolated heparin samples were separated into two species, slow moving and fast moving heparin by agarose gel electrophoresis. The anticoagulant activity of heparin samples was determined by using assay of Mitali et al [1982]
Results: The percentage yield of heparin extracted by Volpi method was significantly higher [P<0.001] i.e. 0.446% with the anticoagulant activity of 19 IU/ mg for bovine pancreas
Conclusion: Heparins are important in the field of medicine and pharmaceuticals. The results of our study showed that heparin isolated by the method of Volpi was significantly higher for bovine pancreas
ABSTRACT
Background: In Pakistan malaria is prevalent in different parts of all the four provinces. The estimated number of annual malaria cases in Pakistan is 1.5 million. The climate of Pakistan is conducive for malarial transmission
Objective: The present study was planned to determine the laboratory surveillance of malaria in Bahawalpur District over a period of five years
Patients and Methods: The present descriptive study was conducted on malarial epidemiology in Bhawalpur region over a period of 5 years from 2007 to 2011. The study was conducted by collecting data from those health facilities where malaria screening was done routinely. Thick and thin smears were taken by the trained personal on glass slides and stained by Giemsa technique. They were then examined microscopically for the diagnosis and specification of malarial parasite. Doubtful samples were sent to consultant haematologists at tertiary level health facilities for confirmation
Results: Annual parasite index was highest in 2007 i.e. 0.091. Slide positivity rate was highest [0.21] in 2007 and dropped [0.01] in 2010. Annual Blood Examination Rate was also highest [4.28] in 2007
Conclusion: Our findings showed that annual blood examination rate, slide positivity rate and annual parasite index continued to drop during the study period