ABSTRACT
The objective of the current study was to evaluate the effects of nandrolone decanoate in terms of morphological changes in the diaphyseal region of appendicular skeleton long bone [femur] of aging female Albino rats with the help of innovatively designed histomorphometric tool of study being introduced first time mapped out micro porosities [lacunae] which tend to intensify as part of advanced aging denoting deterioration in osseous tissue. In fact our research trial has been in the line of involutional osteoporosis especially common in post-menopausal aged women, as to find out appropriate drug usefulness in the care and cure of this diseased state as per recommendation of FDA. An animal experimental research trial was done in strict accordance with the guidelines of the Institutional Animals Ethics Committee[IAEC] and all standard dietary protocols were observed .Rats were separated in two groups comprising of five rats in each. Group A was control and group B was nandrolone decanoate treated 3mg/kg body weight I/M on daily basis for four week duration. On completion of treatment animals were sacrificed, dissection was done and bones were excised. Qualitative histological assessment was worked through SEM, where as the Quantitative data was constructed through a special company fitted software in electron microscope, model "jeol JSM-6380".,the procured data was evaluated statistically and high degree significant variation was detected b/w control and experimental groups. The histomorphometry validated relatively reduced index of BLD in experimental/treated case signifying less resorptive activity in aging bones with the use of nandrolone decanoate
ABSTRACT
To compare the histomorphometric data [in line with osteoporosis] between Tamoxifen treated and Nandrolone decanoate treated female Albino rats through an innovatively modified parameter using SEM [scanning electronic microscopy]. An animal experimental research study was done in which the subjects were ageing six female Albino rats, 8-12 months of age. The animal study was carried out in strict accordance with the guidelines of the Institutional Animals Ethics Committee [IAEC] and all standard dietary protocols were observed. The Albino rats were divided into two main groups comprising of three rats in each group. Group [A] was Tamoxifen treated, 5 mg/kg body weight s/c daily and Group [B] Nandrolone decanoate treated 3 mg/kg body weight, I/M per week. Both groups were treated for six weeks. On completion of treatment the animals were sacrificed, dissected and bones were removed. Quantitative and qualitative data was developed through SEM. The histomorphometric data in this study was Specific Bone Surface [SBS] Specific Eroded Surface [SES] and Total Bone Surface [TBS]/Total Eroded Surface [TES]. Comparison of these parameters was done by the SEM for the two groups of Albino rats i.e. group A; Nandrolone decanoate treated and group B; Tamoxifen treated. The data obtained was evaluated statistically and no significant variation was found between the two groups. The histomorphometric data shows that the effects of Nandrolone decanoate and Tamoxifen in female Albino rats are similar in terms of structural measurement such as SBS, SES and TBS/TES
Subject(s)
Animals, Laboratory , Nandrolone/analogs & derivatives , Tamoxifen , Microscopy, Electron, Scanning , Rats , Bone DensityABSTRACT
Administration of quinolone therapy is controversial during growing age as stated by earlier authors. The flouroquinolones are currently not indicated for young children because of arthropathy and adverse effect as new born shown by studies. However the effects of ciprofloxacin and ZnCl[2] on prenatal conceptus have remained undocumented. The present study was designed to compare the effects on conceptus after maternal ingestion of ciprofloxacin and ZnCl[2] using Wastar albino rats. Ciprofloxacin and ZnCl[2] was administrated to pregnant female albino rats. Ciprofloxacin with a dose of 20 mg/Kg bodyweight and ZnCl[2] 120 microg/100 gm bodyweight two times therapeutic dose for 10 days [from day 8-18 of pregnancy]. Each animal was weighted on day 1, day 8 and day 18 of pregnancy. Abortion resulted on day 18[th] of pregnancy. Each group of pregnant animals were sacrificed on day 18 of gestation by over dose of either anaesthesia, abdomen opened, uterus and both cornua containing conceptus identified, removed, there weight recorded, crown rump length was measured and was compared with similar value of control animals. The results were statistically analysed to find out the significance. The ciprofloxacin induces a mordanting effect as obviated by increased basophilia. Our study reveals that ciprofloxacin administered in maternal, decreased maternal body weight to 38.4 +/- 0.9 gm. However simultaneous ZnCl[2] maintained the body weight to 41.4 +/- 0.7 gm, while ZnCl[2] increased the body weight to 46.5 +/- 2.25 gm. The body weight and Crown Rump length [CR Length] in conceptus decreased by 4.52 +/- 0.10 gm and 3.06 +/- 0.09 Cm respectively. That ciprofloxacin and ZnCl[2] administration maintained the body weight and CR length by 5.46 +/- 0.09 gm and 3.79 +/- 0.13 Cm respectively. That ZnCl[2] administration increased the body weight and CR length by 6.71 +/- 0.05 gm and 4.15 +/- 0.08 Cm respectively. Prenatal administration of Ciprofloxacin caused reduction in growth rate and CR length, and ZnCl[2] maintained body weight and CR length and growth of the rat conceptus.
ABSTRACT
Present study aimed to investigate some free-living medium-sized and large mammals of Chotiari Reservoir and its environs, District Sanghar, Sindh. Mammals belonging to two orders viz. Carnivora and Artiodactyla were studied. Main reservoir, surrounding lakes, marshes, irrigated canals, cultivated lands, sand dunes, southern edges of Thar Desert and human habitations were studied as mammalian habitats. Evidences concerning thirteen species of medium-sized and large mammals were obtained from the complex. The results include data on species richness, abundance and distribution, organized into species account. Species thought to have become extinct include Indian wolf and striped hyena. Hog deer is confined to privately owned game reserves with no more wild population. Only four males of introduced Chinkara still survive in an isolated island within the reservoir. Rare species of the area include smooth-coated otter, Indian desert cat, caracal and fishing cat