ABSTRACT
Objective: to see the effect of different solutions and storage temperature on morphological preservation of rabbit kidney and liver tissues
Material and Methods: in this experimental study, rabbit kidney and liver slices were stored in Ringer Lactate, Euro Collins and University of Wisconsin solutions [UW solution], for various fixed time intervals. The three solutions were tried at room temperature as well as at 0-4degreeC. Morphological preservation was assessed by a semi quantitative method
Results: adequate morphological preservation of both tissues was obtained in all solutions at room temperature for the first 24 hours. Lowering of storage temperature to 0-4degreeC was highly beneficial to both tissues in all solutions. UW solution is more effective for liver tissue preservation
Conclusion: anyone of the solutions may be used at room temperature, for preservation of tissue to be submitted for immuno-histochemistry for the first 24 hours. If prolonged storage is required, lowering of temperature is recommended
ABSTRACT
Formalin, the most widely used preservative, has certain drawbacks. The foremost of them is interference with immunohistochemistry. To evaluate the efficacy of three commonly used organ preservation solutions for tissue preservation this experimental study was carried out on rabbit kidney and liver The tissue slices were stored in Ringer Lactate, Euro Collins awl University of Wisconsin solutions [UW solution], for various fixed time intervals. The three solutions were used in their original form as well as with addition of antimicrobial agents. Morphological preservation was assessed by a semi quantitative method. Kidney slices showed better morphological preservation than liver tissue. UW solution demonstrated the highest preservation of the three solutions tried. UW solution was especially effective for liver preservation. Addition of anti microbial agents was more beneficial to kidney preservation than liver. Thus it was concluded that organ preservation solutions may be used for tissue preservation as well
Subject(s)
Animals, Laboratory , Organ Preservation/methods , Kidney , Liver , Rabbits , Isotonic Solutions , Hypertonic SolutionsABSTRACT
Formalin though an excellent preservative of tissue morphology for routine use, compromises tissue antigenicity. This interferes with immunohistochemistry. An attempt was made to preserve rabbit renal tissues in three most commonly used organ preservation solutions. These are Ringer lactate, Euro Collins solution and University of Wisconsin solution. The tissue slices were kept in these solutions at room temperature for various fixed time intervals. This was followed by routine processing. The degree of preservation was assessed in qualitative as well as quantitative terms. The quantitative assessment was made by a newly devised method. This semi quantitative method is based on established microscopic features of autolysis. The method was found to be reliable and reproducible. Amongst the solutions tried, UW solution revealed clear superiority over other solutions