ABSTRACT
Introduction:Placenta is a mirror which reflects the antepartum status of fetus in utero. Our present study aimed to analyse the morphometry of placenta in normal and assisted reproduction.Subjects and Methods:30 Placentas of natural conception and 42 placentas of assisted reproduction (18 cases of singleton pregnancies, 12 cases of twin pregnancies) were collected from OG department, Rajah Muthiah Medical College and from private fertility centre in and around Chidambaram immediately after delivery. Morphometric dimensions of placenta like thickness, diameter, area, number of cotyledons were measured placental weight, fetal weight were also measured.Results:Placental weight of natural conception was 489 ± 107.84 grams, 316.78 ± 88.24 grams in assisted reproduction. Thickness of placenta was 2.767 ± 0.68 cm in natural conception, 1.78 ± 0.68 cm in assisted reproduction. Number of cotyledons, fetal weight, feto - placental ratio were also reduced in assisted reproduction.Conclusion:This is the first study to analyse the morphometric dimensions of placenta in natural conception and in assisted reproduction. Overall there was a reduction in all dimensions of placenta in assisted reproduction. This will be useful for pediatricians who handle the newborns of assisted reproduction.
ABSTRACT
Background: In the medical field, the trend of the surgical branches has moved towards minimal invasivesurgeries for reasons of decreased morbidity and mortality. To achieve this is thorough knowledge of the anatomy,with the variations of the concerned structure is required. Hence for a structure like superior mesenteric artery,the anatomic variations of its origin and branching pattern is important for accurate interpretation in diagnosticimaging, as well as in deciding the optimum elective procedure in surgical radiological, and interventionalmanagement.Materials and methods: The study was carried out in 50 well embalmed cadavers of South Indian origin irrespectiveof age and sex. Variations in the branches of superior mesenteric artery were noted.Results: Inferior pancreaticoduodenal artery arose from the first jejunal artery. Middle colic and right colicarteries arose as common trunk. The right colic artery was absent. Ileocolic artery arose as common trunk withright colic artery.Conclusion: A thorough knowledge of the anatomy of colonic mesenteric arteries is necessary to accomplishsuccessful uncomplicated abdominal operations, especially laparoscopic colonic resection for cancer usingproximal vascular ligation and wide-en-block resection in which the mesenteric vessels cannot be palpated.
ABSTRACT
The present research work was carried out to observe the effect of various concentrations of Benzyl amino purine (BAP) in terms of multiple shoot induction with special emphasis on qualitative and quantitative changes of proteins during multiple shoot formation. From whole embryonated cotyledons of groundnut. The shoot induction medium containing different concentrations of Benzyle amino purine ranged from 5.0 to 25.0mg/l along with lower concentration of Indole acetic acid (IAA).The multiple shoots were observed in all the BAP concentrations at a varying levels. Among the various levels of BAP, 25.0mg/l of BAP plus 0.50mg/l of IAA was found to be the most effective in terms of multiple shoot induction. The growth parameters like plantlet height, fresh and dry weight also highly influenced by the concentration of the BAP. The root induction was achieved in micropropagated shoots by using Indole butyric acid (IBA) at a concentrations of 1.0 to 5.0mg/l. The mean number of roots, root length was highly influenced by IBA. In all the concentrations of IBA the rooting was associated with moderate and heavily associated basal callusing. The quantitative estimation of protein content in regenerated shoots also increased with higher concentrations of BAP. The SDS-PAGE analysis showed that there was a nine visible bands with 82.2, 76.4, 57.5, 27.6, 23.7, 21.4, 18.2, 16.5 and 12.8 kDa were observed in multiple shoots derived from all the concentrations of BAP. Whereas in whole embryonated cotyledonary explants there was a entirely different protein banding