ABSTRACT
To evaluate the renal sodium-potassium adenosine triphosphatase [Na+/K+ATPase] activity, kidney morphology, and the probable protective effects of 2 different anesthetic agents used during pneumoperitoneum [PP]. The study was performed at Gazi University Experimental Research Center, Ankara, Turkey between January and July 2009. Twenty-four Wistar albino male rats weighing 320-380 g were randomly allocated to 4 groups after receiving ethics committee approval. All rats were cannulated, intubated, and ventilated under ketamine anesthesia. No further surgical intervention was performed for group I. An intraabdominal pressure [IAP] of 10 mm Hg was created by CO[2] insufflation in 18 animals for one hour. The animals in group II received no further anesthetic agents, while the animals in groups III and IV received propofol and sevoflurane. At the end of the protocol, all animals underwent left nephrectomy without sacrificing. Urine was collected from each animal for the following 24 hour for the evaluation of urine creatinine and protein. The activity of renal Na+/K+ATPase was significantly lower in groups II [p=0.014], III [p=0.019], and IV [p=0.032] compared to group I. The pathological score was significantly higher in groups II [p=0.017], III [p=0.028], and IV [p=0.039] compared to group I. No statistically significant difference was found among groups II, III, and IV in terms of Na+/K+ATPase activity and pathological scores. Elevated IAP is related with impaired kidney functions and morphology, and the so-called renoprotective agents neither improved, nor worsened PP-related renal impairment
ABSTRACT
To investigate the effects of lidocaine on the morphology of saphenous veins [SVs] harvested during coronary artery bypass graft [CABG] surgery. This experimental study was conducted at the Cardiovascular Surgery Department, Gazi University, Ankara, Turkey, between May and September 2007. The SVs from 11 patients who underwent CABG surgery were divided into 3 segments. Each segment from the same location of the grafts was allocated into 3 groups as control group [group C], physiologic saline group [group PS], and lidocaine group [group L]. Nitric oxide synthase [NOS], nitric oxide [NO] pool, super oxide dismutase [SOD], and thiobarbituric acid reactive substances [TBARS] levels were measured in the samples from the groups. Histologic specimens were evaluated according to previously defined criteria, and scored accordingly. Histological examination of the grafts in groups L and C were similar, but histological scoring of grafts in group PS were statistically higher than group C [p=0.008]. Nitric oxide synthase activity and NO pool were higher in groups L and PS than in group C [p=0.010]. Super oxide dismutase activity was higher in group L than in group PS [p=0.008]. Super oxide dismutase activity was lower in group PS than in group C [p=0.047]. There was no significant difference between TBARS level in all groups. Our results indicate that primary damage might occur during surgery due to traumatic handling of the graft, and succeeding injuries could occur due to ischemia-reperfusion injury during the waiting period. Adding lidocaine to the preservation solution will protect later injury