Modulating heat shock proteins 70 and 90 expression by low power laser irradiation [635nm and 780nm] in jurkat E6.1 T-lymphocyte leukemia cell line

Heat shock proteins [HSPs] are molecular chaperones involved in protein folding, stability and turnover, and due to their role in cancer progression, the effect of low power laser irradiation [LPLI] on the expression of HSP70 and HSP90 in Jurkat E6.1 T-lymphocyte leukemia [JELT] cell line was investigated in vitro. JETL cells were irradiated with LPLI at 635nm and 780m wavelengths [energy density 9.174 J/cm[2], and assessed for the expression of HSP70 and HSP90 by flow cytometry after 24, 48 and 72 incubation time periods [ITPs]. At 24 hours ITP post-irradiation, control cultures showed that 10.7% of cells expressed HSP70, while LPLI cultures at 635nm and 780nm manifested a higher expression [32.1and 21.3%, respectively], and the difference was significant [P

Control of acute variceal haemorrhage a comparison of octereotide and baloon temponade

A multicenter prospective randomised control trial of patients with acute bleeding from oesophageal varices. Patients not fit for immediate sclerotherapy or band ligation because of severe torrential haemorrhage. This study was conducted at three centers of District Sialkot, i.e., Govt. Sardar Begum Hospital, Allama Iqbal Memorial Hospital, Memorial Christian Hospital from March 1999 to April 2002. The objective was to compare the efficacy and practicality of two commonly used techniques Octeriotide vs Balloon Temponade [BT] for the control of acute variceal haemorrhage. A total of 86 patients were studied in two groups, 46 in Balloon Temponade and 40 in Octeriotide infusion group. These patients presented with acute variceal bleeding. The diagnosis was confirmed by upper GI endoscopy. Stoppage of bleeding was achieved in 44 of 46 patients. Efficacy was 95.65% in Balloon group whereas this was achieved only in 37 of 40 patients. Efficacy was only 85% in Octeriotide group and in BT group complete stoppage of bleeding was seen in 40 [86.96%] whereas minor ooze present in 6[13.04%] at the end of 12 hours. In octeriotide group 25[62.50%] patients achieved complete stoppage of bleeding in [30%] and minor ooze was present, in 3 [7.5%]. No effect on bleeding after 12 hours infusion at 50 ug/hour. Balloon Temponade is the only certain way to control acute profuse variceal haemorrhage. This technique is especially valuable during the transport of patients. The efficacy of octeriotide is limited only to minor/moderately severe cases of variceal bleeding or in combination with balloon Tamponade when patients are bleeding from gastric' fundal varices or from portal hypertensive gastropathy