Comparison of the response using ICR mice derived from three different sources to multiple low-dose streptozotocin-induced diabetes mellitus / 한국실험동물학회지

This study was conducted to compare the multiple low-dose streptozotocin (MLDS)-induced diabetic patterns of Korl:ICR, A:ICR, and B:ICR mice obtained from three different sources. Six-week-old male ICR mice were obtained from three difference sources. Korl:ICR mice were kindly provided by the National Institute of Food and Drug Safety Evaluation (NIFDS). The other two groups of ICR mice were purchased from different vendors located in the United States (A:ICR) and Japan (B:ICR). All ICR mice that received MLDS exhibited overt diabetic symptoms throughout the study, and the incidence and development of diabetes mellitus were similar among the three ICR groups. The diabetic mice exhibited hyperglycemia, loss of body weight gain, decreased plasma insulin levels, impaired glucose tolerance, decreased number of insulin-positive cells, and decreased size of β-cells in the pancreas. The diabetes symptoms increased as the blood glucose level increased in the three ICR groups. In particular, the level of blood glucose in the STZ-treated group was higher in Korl:ICR and A:ICR mice than in B:ICR mice. The plasma insulin levels, glucose tolerance, blood chemistry, and morphological appearance of the pancreas were very similar in the ICR groups obtained from the three different sources. In conclusion, our results suggest that Korl:ICR, A:ICR, and B:ICR mice from different sources had similar overall responses to multiple low-dose STZ to induce diabetes mellitus.

Surgical treatment of mucin-producing cholangiocarcinoma arising from intraductal papillary neoplasm of the intrahepatic bile duct: a report of 2 cases

Intraductal papillary neoplasms of the bile duct (IPNB) leads to malignant transformation and mucin production. Herein, we presented two cases of mucin-producing IPNB with obstructive jaundice who underwent resection of the intrahepatic lesions and bypass hepaticojejunostomy. The first case was a 69 year-old male patient with 5-year follow up for gallstone disease. Imaging studies showed mucin-secreting IPNB mainly in the hepatic segment III bile duct (B3) and multiple intrahepatic duct stones for which, segment III resection, intrahepatic stone removal, end-to-side choledochojejunostomy and B3 hepaticojejunostomy were conducted. The second case was a 74 year-old female patient with 11-year follow up for gallstone disease. Imaging studies showed mucin-producing IPNB with dilatation of the segment IV duct (B4) and mural nodules for which, segment IV resection, partial resection of the diaphragm and central hepaticojejunostomy were conducted. Both patients recovered uneventfully from surgery. These cases highlight that in patients with IPNB, abundant production of highly viscous mucin inducing obstructive jaundice may be associated with malignant transformation.

Antioxidant and hepatoprotective effects of fermented red ginseng against high fat diet-induced hyperlipidemia in rats / 한국실험동물학회지

This study was performed to investigate the antioxidant and hepatoprotective effects of fermented red ginseng (Panax ginseng C.A. Meyer; FRG) on high-fat diet-induced hyperlipidemia in rats. Sprague-Dawley rats were divided into four groups of seven: normal control, NC; high-fat diet control, HFC; high-fat diet–0.5% FRG, HF-FRGL; and high-fat diet–1% FRG, HF-FRGH. All rats were fed a high-fat diet for eight weeks, except those in the NC group, while rats in the FRG treatment groups received drinking water containing 0.5% or 1% FRG. After eight weeks of treatment, levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglycerides (TG), low-density lipoprotein-cholesterol (LDL-C), and high-density lipoprotein-cholesterol (HDL-C) in the serum were measured. The concentration of the oxidative stress marker malondialdehyde (MDA), and activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in rat liver were evaluated. Histological analysis of the liver was performed using hematoxylin and eosin. The high-fat diet markedly increased serum levels of ALT, AST, TC, TG, and LDL-C and hepatic MDA levels, while administration of FRG to the hyperlipidemic rats resulted in a significant decline in the levels of these parameters. Furthermore, the decline in the levels of serum HDL-C and hepatic SOD, CAT, and GSH-Px induced by the high-fat diet was attenuated by FRG treatment. In addition, histopathological analysis of liver sections suggested that FRG treatment also provided protection against liver damage. These results suggested that FRG improved lipid profiles, inhibited lipid peroxidation, and played a protective role against liver injury in hyperlipidemic rats.

Customized left-sided hepatectomy and bile duct resection for perihilar cholangiocarcinoma in a patient with left-sided gallbladder and multiple combined anomalies

Left-sided gallbladder (LSGB) is a rare anomaly, but it is often associated with multiple combined variations of the liver anatomy. We present the case of a patient with LSGB who underwent successful resection of perihilar cholangiocarcinoma. The patient was a 67-year-old male who presented with upper abdominal pain and obstructive jaundice. Initial imaging studies led to the diagnosis of Bismuth-Corlette type IIIB perihilar cholangiocarcinoma. Due to the unique location of the gallbladder and combined multiple hepatic anomalies, LSGB was highly suspected. During surgery after hilar dissection, we recognized that the tumor was located at the imaginary hilar bile duct bifurcation, but its actual location was corresponding to the biliary confluence of the left median and lateral sections. The extent of resection included extended left lateral sectionectomy, caudate lobe resection, and bile duct resection. Since some of the umbilical portion of the portal vein was invaded, it was resected and repaired with a portal vein branch patch. Due to anatomical variation of the biliary system, only one right-sided duct was reconstructed. The patient recovered uneventfully without any complication. LSGB should be recognized as a constellation of multiple hepatic anomalies, and therefore, thorough investigations are necessary to enable the performance of safe hepatic and biliary resections.

A comparison of hemodynamics in elderly patients that were administered thiopental sodium or propofol to induce general anesthesia / 대한마취과학회지

BACKGROUND: Thiopental sodium and propofol are commonly used to induce anesthesia. This study was conducted to compare the hemodynamic effects of propofol and thiopental sodium during the induction of general anesthesia in elderly patients. METHODS: Forty patients undergoing general anesthesia were randomly divided into two groups. In group T, thiopental sodium was used to induce anesthesia, whereas propofol was used in group P. Hemodynamic changes in the mean blood pressure, heart rate, cardiac index (as determined using a NICCOMO monitor) and bispectral index (BIS) during induction were then compared between the two groups. In addition, the dosage of induction agent, time required until loss of response to verbal order (LOV) and eyelid reflex were compared between groups. RESULTS: The mean blood pressure was significantly lower in group P than in group T during the 1-5 min following LOV and during the 2, 3 min following intubation (P < 0.05). However, the heart rate did not differ significantly between the two groups. The cardiac index also did not differ significantly between groups, except during the first 3 min following LOV. Finally, the BIS value were significantly lower than the baseline values in both groups during induction. CONCLUSIONS: In elderly patients, the heart rate, cardiac index and BIS value did not differ significantly between the two groups. But the mean blood pressure was significantly lower in propofol than in thiopental sodium.

Effective removal of epistaxis during nasotracheal intubation utilizing a fiberoptic scope in a difficult airway: A case report / 대한마취과학회지

Nasal bleeding related to nasal trauma is the most common complication of nasotracheal intubation with a fiberoptic scope. When nasotracheal intubation with a fiberoptic scope is performed, profuse bleeding from the nasal cavity makes it difficult to handle a fiberoptic scope and may cause a hypoxemia. So when nasal bleeding occurs, it is important to suction the nasal cavity for easy handling of a fiberoptic scope. But, it may be difficult to suction in a nasal cavity with a fiberoptic suction port only. We report a patient with nasal bleeding following nasotracheal intubation with a fiberoptic scope because of articular trismus, and successful nasotracheal intubation utilizing a fiberoptic scope by suctioning with an extra suction apparatus.

Effective removal of epistaxis during nasotracheal intubation utilizing a fiberoptic scope in a difficult airway: A case report / 대한마취과학회지

Nasal bleeding related to nasal trauma is the most common complication of nasotracheal intubation with a fiberoptic scope. When nasotracheal intubation with a fiberoptic scope is performed, profuse bleeding from the nasal cavity makes it difficult to handle a fiberoptic scope and may cause a hypoxemia. So when nasal bleeding occurs, it is important to suction the nasal cavity for easy handling of a fiberoptic scope. But, it may be difficult to suction in a nasal cavity with a fiberoptic suction port only. We report a patient with nasal bleeding following nasotracheal intubation with a fiberoptic scope because of articular trismus, and successful nasotracheal intubation utilizing a fiberoptic scope by suctioning with an extra suction apparatus.