Comparative study on detection of urease activity by CLO Test Kit and Microtiter Biopsy Urease Test Kit for evaluation of Helicobacter pylori

A Microtiter Biopsy Urease Test (MBUT) for the detection of Helicobacter pylori was evaluated in the drug trial study conducted in the Endoscopy Department of New Yangon General Hospital. The MBUT was compared with commercially available urease test (CLO Test). Both CLO test and MBUT were performed on antral and fundal gastric tissues biopsied from 35 subjects who underwent upper gastrointestinal endoscopy. 29 (83 percent) cases were positive for Helicobacter pylori when tested by CLO test and MBUT. Thus the sensitivity and specificity of MBUT relative to CLO test was both 100 percent respectively. Tested by MBUT, the urease reaction was positive in 83 percent of cases within 2 hours and 90 percent within 3 hours. Tested by CLO test, it was positive in 60 percent of cases within 2 hours and 67 percent within 3 hours. the accuracy of MBUT was thus similar to that of CLO test for the detection of Helicobacter pylori. Moreover, MBUT is not expensive, can be prepared locally, packed in test kit form and can be supplied to gastroenteroscopy units for diagnostic and treatment purposes.

Role of vegetable protein in maternal nutrition in wistar rats

This research was performed in the Department of Medical Research, Yangon, Myanmar. The experimental work was done during the period of April 1987 to January 1988.This paper is part of a thesis submitted in partial filfilment for the degree of Master of Medical Science (Physiology) by Dr. Hla Hla Aye. It was read at the Research Paper Reading Session, the Twenty-Fifth Anniversary of the Department of Medical Research on the 31st December 1988.

The effect of "Third Trimester" maternal nutrition on the offspring in Wistarrats

This research was performed in the Department of Medical research, Yangon, Myanmar. The experimental work was done during the period of April 1987 to 1988. This paper is part of a thesis submitted in partial fulfillment for the degree of Master of Medical Science (Physiology) by Hla Hla Aye. It was read at the Research paper Reading Session, Twenty-Fifth Anniversary of the Department of Medical Research, on 31st December, 1988.

A study of glucose and insulin profiles in acute viral hepatitis patients

Oral glucose tolerance tests, with 75 gm of glucose load were carried out on 25 patients suffering from acute viral hepatitis including 15 pregnant women and on 25 apparently normal subjects. the mean fasting blood glucose level of hepatitis patients (62.3 - 2.6 mg/dl) was found to be significantly (p<0.01)lower than that of normal subjects (73.6 - 2.0mgdl). After drinking the glucose solution hepatitis patients generally had higher blood glucose levels compared to normal sbjects. Blood glucose and plasma insulin concentration were measured in another 35 acute viral hepatitis patients after an overnight fast, to study the relation between these values and the parameters of liver Function Test. The fastion blood glucose level was found to be inversely correlated to SGOT and SGPT level in these patients.

The growth stimulating factor in parabiotic rats

Littermate Wistar rats were parabiosed at 30 days and daily individual food intake and individual weight gain of fifteen parabiotic pairs were recorded as control parabiotic pairs. Another fifteen pairs were used for the experiment in which one partner of parabiotic pair was completely fasted for 4 days and then refed ad libitum till body weight returned to the prefasting level and throughout the experiment, individual body weight changes and individual food intake of both partners of test parabiotic pairs were recorded. During fasting period, daily food intake and weight gain of non-fasted partner of test parabiotic pair increased significantly over that of the individual rat in control parabiotic pair. Moreover, weitht loss of fasted partner is same as that of individual fasted rat. The above findings indicated the presence of factor/factors in plasma of fasted partner stimulating food intake and growth of non-fasted partner of the test parabiotic pair. The present study also reveals that there is greater body weight increase per. g. food consumed (food conversion efficiency) of fasted partner during refeeding period whereas that of non-fasted partner decreased significantly during that period. Food intake and factors controlling it have been extensively studied by Physiologists and Nutritionists interested in growth and its regulation. Various extenal and endogenous stimuli initiate and terminate feeding. Of the endogenous stimuli humoral factors and their mode of action are the subjects of several recent studies (1,3,4,6). The present study is undertaken in order to further clarify and assess the role of humoral factor in the regulation of food intake and growth.