ABSTRACT
Microbial contamination of dental unit surfaces and materials are of considerable importance since patients and dental staff is regularly exposed to aerosols generated from the dental unit and contaminated surfaces. The aim of this study was to investigate the effect of BIB forte in disinfection of dental instruments. In this study we assessed the composition of aerobe and facultative anaerobe bacterial micro flora before and after administration of BIB forte. In this experimental study, 30 microbial samples were obtained from dental instruments before administration of BIB forte and were subjected to microbiological evaluations. The samples were cultured on the specific media and number of the bacterial colonies was counted after keeping at 37 degree centigrade for 24 hours. The effect of BIB forte was also investigated on standard lab species and the number of formed colonies was counted. Data were analyzed by SPSS; Chi-square, and Fisher's exact tests were used. 93% of all species samples were positive regarding presence of bacteria. Microorganisms isolated were mainly composed of: staphylococcus aureus, streptococcus, corynebacterium, Neisseria and Bacillus species. BIB forte significantly reduced the microbial contamination after applied on dental surfaces and prevented the growth of standard microorganisms' in-vitro. BIB forte is effective in reducing the bacterial load to an acceptable level and its usage is recommended as an ideal disinfectant in routine dental practice