Histological Evaluation of Experimentally Induced Critical Size Defect Skin Wounds Using Exosomal Solution of Mesenchymal Stem Cells Derived Microvesicles

BACKGROUND AND OBJECTIVES: The present study investigated whether MSCs derived microvesicles (MVs) or (Exosomes) can exert therapeutic effects on an experimental model of cutaneous injury and explored the underlying involving mechanisms. METHODS AND RESULTS: Three bilateral full thickness circular wounds were created on the back of two groups of dogs using 2-cm dermal punch. The wounds were at least 2.5 cm apart. Saline was subcutaneously injected in 4 places around each wound area in group-I (control), whereas an equal volume of exosomal solution of MSCs derived MVs was similarly injected in group-II. The findings demonstrated that MSCs derived MVs had significantly promoted cutaneous wound healing, collagen synthesis, and vascularization at wound sites. The application of the exosomal solution had not only promoted the generation of newly formed vessels, but also have accelerated their development and maturation leading to a faster healing process. CONCLUSIONS: MSC-Exosomes appeared to be a superior candidate for treating cutaneous wounds than their originator cells, and may represent a promising opportunity to develop a novel cell-free therapy approach that might overcome the obstacles and risks associated with the use of native or engineered stem cells transplantation therapy.

Serum uric acid in primary nocturnal enuresis

A case control study was conducted on 41 cases of primary nocturnal enuresis and 20 controls in order to evaluate the implications of serum uric acid in the pathogenesis of primary nocturnal enuresis. Serum uric acid level was significantly lower in cases when compared with controls. No significant difference was detected in serum uric acid level between enuretic children with or without behavioral disturbance. Also, no difference was detected between those considered deep sleepers by their parents or not. This relatively lower level of serum uric acid in children suffering from primary nocturnal enuresis may be a possible factor in the pathogenesis of this problem