Flow cytometric immunophenotyping of bone marrow in cats diagnosed with hematopoietic disorders

Cytological examination of bone marrow in cats, due to the large number of cells and various growth phases is somewhat complicated. The use of flow cytometric techniques and monoclonal antibodies are appropriate methods in the diagnosis of hematopoietic malignancies. The purpose of the present study is to determine cell-surface antigens for various developmental stages of feline bone marrow cells in hematopoietic disorders using flow cytometric. In this study, bone marrow cells from 4 cats with hematopoietic disorders and 2 clinically healthy cats, were labeled with 5 types of anti-feline MAbs included: CD21-like [Cr-Br], T lymphocyte subpopulation, CD-172a, Granulocyte, Pan-Leukocyte [CD45-like] and then analyzed using flow cytometric. The results revealed changes in immunophenotyping and light scatter properties compared with normal cases. The percentage of CD45, Granulocyte and CD172a markers in the bone marrow of a cat with erythroleukemia were lower compared with normal bone marrow. In a cat with myelodysplastic syndrome, scatter plot indicated an increase in the immature myeloid cells and a decrease in mature myeloid cells. It was concluded that cytological examination of bone marrow with studying dispersion studies on cells using flow cytometric and usage of a panel of antibodies such as CD21-like[Cr-Br], T lymphocyte subpopulation, CD-172a, Granulocyte, Pan-Leukocyte [CD45-like] could support the diagnosis of feline hematopoietic abnormalities

Evaluation of therapeutic effects of a 5-day intrauterine infusion of 6% Eucalyptus globulus oil solution on mares with experimentally induced endometritis with Streptococcus zooepidemicus

Endometritis is one of the major causes of infertility in mares. The present study was conducted to evaluate intrauterine infusion of Eucalyptus globulus oil as treatment for mares with experimentally induced endometritis. Mares [n=8] were synchronized using two injections of PGF 2alpha [10 mg]14 days apart. All mares were inoculated with a live culture of 5 × 10[6] CFU S. zooepidemicus in the dioestrus phase after the termination of oestrus. The day of inoculation was considered as day 0 of the experiment. On days 3, 6 and 9, cytological and bacteriological specimens were collected to determine endometritis. Mares were randomly assigned to two groups on day 10. Mares in treatment [n=4] and control [n=4] groups received intrauterine 6% Eucalyptus oil solution [100 ml] and intrauterine saline solution [100 ml] for 5 consecutive days. Cytological, bacteriological, and ultrasonographic evaluations were implemented at days 15, 17 and 19 [1, 3 and 5 days after termination of intrauterine treatment, respectively]. The number of mares diagnosed with endometritis, the depth of their intraluminal uterine fluid and the number of infected mares did not change by the treatment [P>0.05]. In conclusion, the 5-day intrauterine infusion of 6% E. globulus oil solution failed to treat experimental endometritis in mares

[Hematological and serum biochemical parameters in anemic dogs]

Anemia is a common disorder in domestic pet animals, usually encountered in veterinary clinics. The objective of this study was to evaluate the hematological and serum biochemical parameters of the anemic dogs and to show practically the value of measuring the corrected reticulocyte percentage [CRP]. A survey was conducted on 500 dogs which referred to small animal hospital of the faculty of veterinary medicine of Tehran university. From this population, a group of 139 anemic dogs was selected with PCV less than 37% and 20 healthy dogs as control group. They were examined for the hematological parameters [RBC, Hb, PCV, MCV, MCHC, RDW, WBC, PLT, Dif, reticulocyte percentage and corrected reticulocyte percentage] and serum biochemical factors [Fe, alanine amino transferase activity,creatinine, total protein and glucose]. The anemic dogs were divided into 3 anemic groups based on MCV and MCHC: microcytic hypochromic [1.44%], microcytic normochromic [21.59%] and normocytic normochromic [76.97%]. A comparison survey was conducted between the results of the 3 anemic and control groups by one way ANOVA and Tukey test. The measurement of hematological parameters indicated that RBC, Hb and PCV decreased significantly in all anemic dogs. The results showed that RDW of microcytic hypochromic group increased significantly compared to control. The reticulocyte percentage of all anemic groups was significantly different from control group, but calculation of CRP showed there was only significant difference between normocytic-normochromic and control groups. One way ANOVA indicated that serum Fe in microcytic-hypochromic group and serum creatinine in normocytic-normochromic group significantly decreased and increased, respectively. Total protein of all groups is significantly decreased compared to control group. There were no significant changes for the other parameters. Based on the result, it is suggested that CRP and biochemical parameters be measured such as: creatinine, total protein, ALT and glucose in addition to CBC, in all anemic dogs

molecular investigation of widespread Salmonella serovars, S. typhimurium and S. enteritidis, involved in salmonellosis of cattle and sheep in farms around Tehran, Iran

Salmonellosis as an important zoonotic disease that causes food born poisoning in human through animal products and is considered as a worldwide public health hazard. Widespread studies have been conducted on different aspects of incidence, treatment and control of salmonelosis all over the world

The aim of this study was to evaluate the occurrence of widespread Salmonella serovars, S. typhimurium and S. enteritidis, isolated from an outbreak of salmonelosis in cattle herds and sheep flocks around Tehran, in summer 2009, using molecular [PCR and multiplex PCR] and conventional [bacterial culture, serology and antibiogram] tests

Tissue and faecal samples were collected from 8 calves, 5 lambs and 2 aborted cattle embryos. All involved cases were animals less than 2-month-old and the presence of Salmonella serovars were confirmed in all isolates. The infection of S. enteritidis was much more prevalent in comparison to the S. typhimurium, which was statistically meaningfull [P<0.05]

Virulence gene [spv gene] of S. enteritidis was shown on 250 bp fragments in most of the organ isolates. Specifically the virulence gene was shown in all isolates of aborted fetus tissue cultures, through the molecular survey. In two calves, both S. typhimurium and S. enteritidis were detected and S. typhimurium was isolated from liver in both cases

All isolates were sensitive to streptomycin, lincospectin, enrofloxacine and trimetoprim and were resistant to doxycycline and erythromycin?

Isolation of erysipelothrix rhusiopathiae from aborted lambs in Iran: a case report

Ewe abortion and neonatal mortality are serious problems to sheep farmer. The objective of this paper was to report isolation of Erysipelothrix rhusiopathiae from aborted fetuses in a flock in Tehran, Iran. Abortion occurred in 10% of ewes in the flock. The fetuses were well developed and pinpoint, necrotic-like lesions were recorded on the placental cotyledons, whilst the intercotyledonary areas appeared normal. E. rhusiopathiae was isolated in pure culture and heavy growth were seen in abomasumal fluid, lungs, heart and liver of fetuses and vaginal discharge of the aborted ewes. The grown bacteria were identified using conventional bacteriological technique and Erysipelothrix rhusiopathiae was confirmed. On the basis of the available evidence, Erysipelothrix rhusiopathiae might be one of the ovine abortion causes in Iran

Alterations of triglyceride and cholesterol in response to Aloe vera gel extract in HepG2 cells and hyperlipidemic guinea pigs

Aloe vera is well-known for its pharmacological and nutritional properties. The aim of the present study was to determine the effect of A. vera gel extracts on the secretion and cell content of triglyceride [TG] and cholesterol [TC] in HepG2 cells and their short-term effects on the dietary hyperlipidemic guinea pig model. The effects of increasing concentrations of A. vera crude gel and its alcoholic and hydro-extract were compared to HepG2 cells in both basal and TG induced conditions with 20 mM glucose for 24 h. In addition, 24 male guinea pigs were randomly separated into six experimental groups as follows: control, hyperlipidemic control, levostatin control and A. vera receiving groups [fed with lipid-rich diet supplemented with A. vera crude gel, alcoholic or hydro-extracts of A. vera gel]. Treatments were carried out for 10 d TG and TC levels were measured in both collected fluid [sera and media] and extracted tissue [HepG2 and liver]. Although basal and stimulated conditions of crude gel and its hydro-extract decreased the secretion and cell content of TG, compared to the control [p<0.05]. This pattern was not seen with the alcoholic extract. Furthermore, A. vera did not have any effect on the serum or liver contents of TG or TC. Our results suggest that A. vera could be a beneficial supplement to modulate the levels of TG and TC. However, it does not appear to be a short-term lipid modulator for hyperlipidemia

Immune-mediated hemolytic anemia in cats referring to Veterinary Teaching Hospital of Tehran [2006-2007]

Immune-mediated hemolytic anemia [IMHA] is characterized by the destruction of erythrocytes or sometimes bone marrow erythroid precursors mediated by immunoglobulins [IgG, IgM], with or without complement [C[3]]. The main objectives of this study were to assess the laboratory test results of IMHA and to investigate its possible underlying causes in cats referring to the Veterinary Teaching Hospital of Tehran. The Coombs' test [CT] was performed in 74 cats with PCV below 0.35. The test was positive in 26 cats. These positive CT cats were categorized into four groups based on the PCV ranges and type of anemia including: Group A: 9 of 26 cats had nonregenerative anemia [PCV median, 0.22] [1 feline leukemia virus positive which had erythroleukemia, 1 feline infectious peritonitis positive, 1 with Hemoplasma spp., 3 with renal failure, 2 with inflammatory disease and 1 with no diagnosis]. Group B: 4 cats [PCV median, 0.31] had a regenerative anemia with severe dehydration. Group C: the other 9 cats with a normal range of PCV [median, 0.34] involved with various conditions [vaccination, parturition, acetaminophen poisoning, osteoporosis, and renal failure]. Group D: the remaining 4 cats with a marginal range of PCV [median, 0.30] had a history of inflammatory disease and drug therapy. The results of this study indicated that infectious diseases and drug therapy were the main factors associated with positive Coombs' test results

[Molecular identification of mycoplasma agents in suspected contagious caprine pleuropneumonia]

Contagious Caprine Pleuropneumonia [CCPP] is one of the common infections in the middle east regions. So far, there has not been received any report about isolation and identification of these agents in Iran. The aim of this study is to diagnose and isolate mycoplasma agents in suspected goat flocks. Total of 100 pneumonic lung specimen from 20 CCPPsuspected flocks were collected from abbatoirs close to Kermanshah during 1384-1386 and had been sent to Microbiology Lab. Gross lesions showed hepatization with grey and white lesions [consolidation] and motley appearance with or without fibrin. The minced tissue were inoculated to PPLO broth agar. After multiple passages, typical mycoplasma colony was isolated from 4 flocks [22/2%]. Mycoplasma DNA was also extracted based on phenolchloroform method and subjected to generic PCR with specific primers. In addition to the perivious positive samples from tissue culture, 5other flocks also showed contamination with Mycoplasma organisms in PCR tests[45%]. Then, the samples were determined for Mycoplasma mycoides cluster infection, M. capricolum capripneumonia and M. mycoides mycoides [L.C], using M. agalactia as negative control, with specific primers in PCR, there has showed no contamination to these strains. However, to declare "free status " from CCPP in goat flocks requires more developed researches and much more samples in further investigation

Prevalence of bacterial mastitis in cattle from the farms around Tehran

To determine the prevalence of bacterial mastitis in cattle, milk samples positive for California mastitis test [CMT] were cultured during a period of almost 4 years. The bacterial species isolated from 2904 milk samples studied were coagulase negative Staphylococcus sp. in 879 [30.27%] samples, Streptococcus agalactiae in 642 [22.11%], 5. dysgalactiae in 332 [11.43%], E. coli in 295 [10.16%], Staphylococcus aureus in 84 [2.89%], Bacillus cereus in 51 [1.76%], Arcanobacterium pyogenes in 31 [1.07%], Pseudomonas aeruginosa in 6 [0.21%], Klebsiella pneumoniae in 4 [0.14%], Pasteurella multocida in 1 [0.03%] and Mycoplasma sp. in another [0.03%] sample. No growth was found in 578 samples [19.90%]. Thirty-one [37%] of 84 animals which were infected with S. aureus, had acute infection. We found that contamination of milk with coagulase negative staphylococci are the most frequent bacterial infection in dairy cattle around Tehran; it mostly causes subacute form of the disease. S. agalactiae, S. dysgalactiae and E. coli are the second, third and the fourth causative agents

Artificial insemination encountered with haemophilus somnus infection in cattle

During a period of artificial insemination in cattle in several farms around Tehran, Iran, the animals were involved with endometritis and abortion. In order to investigate the cause, bacterial culture was done on 23 samples of artificial prepared semen. Haemophilus sp. was isolated from 20 out of 23 samples and the findings were confirmed through serologic tests. Biochemical tests were also set up to diagnose the strain of the bacteria. These tests revealed the presence of H. somnus in the artificially prepared semen. This species was never before reported in Iran, in genital tract of cattle [male and female] nor H. agni in sheep. Attempts to culture this bacteria from genital tract has not been very successful because of cultural difficulties. This was the first diagnosis of H. somnus in this country and helped the practitioners to be aware of the possible problems in running the artificial insemination procedure from infected semen

[Isolation of Histophilus somni [Haemophilus somnus ] from pneumonic lesions of slaughtered cattle in suburbs of Tehran]

Objective: Isolation of Histophilus somni [Haemophilus somnus] from pneumonic lesions in cattle

Animals: Fifty - two samples of pneumonic lungs from slaughtered cattle

Procedure: During a six -month period, 52 lung samples with pneumonia were collected from slaughterhouses in suburbs of Tehran for isolation of H. somni. Samples were cultured by standard bacteriological methods and isolated bacteria were identified according to Carter and Cole method by biochemical and enzymatic examinations. Antibiotic sensitivity test was performed on H. somni isolates

Results: H. somni was isolated alone or with other bacteria from 4 [7.69%] lungs with pneumonia. Antibiotic sensitivity test showed that the isolated bacteria were sensitive to Ceftioufur [Exenel] and Enrofloxacine but resistant to Trimethoprim, Penicillin and Streptomycin

Conclusion: To the authors' knowledge this is the first report of H. somni isolation from respiratory system of cattle in Iran and further research is necessary about it

Bacterial causative agents of conjunctivitis and keratoconjunctivitis in cattle, sheep and goat and survey of pathogenicity and immunogenicity of Moraxella species

Bacterial culture of eye secretion from bovine and ovine infected eyes was done during a period of three years [1992-1994]. Moraxella bovis [M. bovis] in bovine and Branhamella ovis [B.ovis] in ovine cases were the most isolates. M. bovis was isolated from 72 of a total of 132 bovine cases [54.54%]. The other isolations were staphylococcus sp., streptoccus sp. Pseudomonas aeroginosa and non pathogenic moraxella sp. In sheep and goat B ovis was isolate from 7 cases of a total of 17 [41%] and two cases were non pathogenic moraxella sp. This observation indicates the high incidence of pathogenicity of B. ovis in these animals however the mechanism of bacterial infection is still unknown. Since Chlamydia and rickettsiae are tought to be responsible for primary conjunctivitis two methods of staining were employed to observe these organisms in eye epithelial cell: 1] Macchiavello staining and 2] modified Ziehl Nelson staining. Only two cases of cattle infected eyes showed chlamidial bodies with second staining method