[Chronic lymphocytic leukemia: flow cytometry contribution]

Chronic lymphocytic leukemia is a part of B lympho-proliferative diseases. Clinically, it is an indolent disease thought it has a variable evolution. Our aim is to focus on the importance of flow cytometry in the diagnosis of chronic lymphocytic leukemia. This is a prospective survey conducted in 2005/2006, concerned 13 cases of chronic lymphocytic leukaemia from 42 B-cell chronic lymphoproliferative syndromes. The clinical features were summarized in the data file filled by the practicians. Chronic lymphocytic leukemia is studied in our laboratory by different means such as: hemogramm with a morphological aspects of peripheral lymphocytes, osteomedulhary biopsy myelogram, and immunophenotyping by FC500 [Trade Mark] cytometer[Beckman Coulter]. Within the 13 cases of chronic lymphocytic leukemia, there were 9 male and 4 female having a mean age of 68.5 years old with extremes from 45 to 80 years old. The most important signs found were: polyadenopathy, splenomegaly or cytopenia. In our patients, lmphocytosis was ranged from 5,6 a 136 g/l. The marrow was infiltrated by small lymphocytes in some cases. The cytometric analysis was based on Matutes score. In most cases, the chronic lymphocytic leukemia diagnosis seems to be easy based on the hemogram and immunophenotyping. Actually, treatment of patient with chronic lymphocytic leukemia depends on Rai or Binet classifications; still, it is important to have more a other accurate markers dae to its variable evolution. Flow cytometry is of real help in the diagnosis and the monitoring of residual disease in chronic lymphocyte leukemia

[Biological and molecular aspects of haemophilia A]

Haemophilia A is a recessive constitutional, hereditary or sporadic, transmission hemorrhagic illness bound to the X chromosome and definite by the deficiency of coagualation factor VIII. We propose in this work the molecular and phenotypic aspects of the haemophilia A. The data on the gene and the FVIII protein are reported. The particular case of the inversion of the intron 22 and more lately the inversion of the intron 1 are at the origin of nearly the half of the severel cases of hemophilia A. The other anomalies are represented respectively by the singles substitutions, deletions and insertions. The present diagnosis strategy of the severe form of the illness simplified itself considerably since the description of the common inversion

hereditary bisalbuminemia [about one case]

Bisalbuminemia is an abnormality of the albumin which is characterised with a double band on serum protein electrophoresis. It could be hereditary with genetic mutation, or acquired during overdosage with beta-lactamines or during pancreatic fistula in a serous collection. We present in this letter, a case of bisalbuminemia, found in a 40 years-old patient presenting a with renal insufficiency

Atteintes hematologiques dans la maladie de gaucher de l'adulte

Authors submitte results of blood, bone marrow, spleen, and lymph-node examinations of three cases of Gaucher's disease. The hematological aberrations and same pathogenetic mechanism of disease are included

propos d'un cas d'agranulocytose au paracetamol

It is not known to the general public that Paracetamol can be toxic in the blood. The authors reported one case of agranulocytosis with hepato-toxicity which was noted in a young woman who had taken Paracetamol. The development is favourable. They noted the rarity of this problem, the mechanism by which it appears to be fundamentally immuno-allergic, the characteristic medullar aspect and the diagnostic pitfalls. The laboratory tests done to incriminate the drug are not adequate, but the agranulocytic culture method remains the safest means

[Anti human T cell leukemia lymphoma virus [HTLV1] prevalence in a Moroccan population]

Epidemiological studies suggest African origin of retrouviruses of HTLV group [human T cell leukemia lymphoma virus] which are incriminated in the etiology of adult leukemia and acquired immunodeficiency syndrome [AIDS]. We have studied 610 sera of healthy subjects from different parts of Morocco. The prevalence of antibodies against HTLV[1] in this sample is weak [0,5%] similar of that shown in USA and Europe