ABSTRACT
Chronic lymphocytic leukemia is a part of B lympho-proliferative diseases. Clinically, it is an indolent disease thought it has a variable evolution. Our aim is to focus on the importance of flow cytometry in the diagnosis of chronic lymphocytic leukemia. This is a prospective survey conducted in 2005/2006, concerned 13 cases of chronic lymphocytic leukaemia from 42 B-cell chronic lymphoproliferative syndromes. The clinical features were summarized in the data file filled by the practicians. Chronic lymphocytic leukemia is studied in our laboratory by different means such as: hemogramm with a morphological aspects of peripheral lymphocytes, osteomedulhary biopsy myelogram, and immunophenotyping by FC500 [Trade Mark] cytometer[Beckman Coulter]. Within the 13 cases of chronic lymphocytic leukemia, there were 9 male and 4 female having a mean age of 68.5 years old with extremes from 45 to 80 years old. The most important signs found were: polyadenopathy, splenomegaly or cytopenia. In our patients, lmphocytosis was ranged from 5,6 a 136 g/l. The marrow was infiltrated by small lymphocytes in some cases. The cytometric analysis was based on Matutes score. In most cases, the chronic lymphocytic leukemia diagnosis seems to be easy based on the hemogram and immunophenotyping. Actually, treatment of patient with chronic lymphocytic leukemia depends on Rai or Binet classifications; still, it is important to have more a other accurate markers dae to its variable evolution. Flow cytometry is of real help in the diagnosis and the monitoring of residual disease in chronic lymphocyte leukemia