Detection of Borrelia persica infection in Ornithodoros tholozani using PCR targeting rrs gene and xenodiagnosis

Relapsing fever caused by Borrelia persica, is an acute tick-borne disease which is transmitted by soft ticks of Ornithodoros tholozani to human. Value of PCR and xenodiagnosis for detection of B. persica in O. tholozani ticks was compared. Sixty-four Borrelia- free ticks were fed on infected guinea pigs and used for the experiments. For xenodiagnosis, a group of 32 ticks in subsequent blood meal were fed on sterile guinea pigs and the indication of B. persica in the animal blood was tested 5-14 days later by dark-field microscopy. For PCR, all 64 ticks were subjected to PCR against B. persica rrs gene [16S-rDNA]. Also sensitivity of PCR in terms of minimum detectable number of spirochetes as well as the effects of tick sex and post digestion was tested. PCR revealed B.persica DNA in 98.4% ticks, in which B. persica were found in 25.0% by xenodiagnosis. PCR was enough sensitive to give positive results for DNA of 1 spirochete. PCR success rates were similar for male or female ticks. Course of time did not affect the efficacy of PCR and similar results were observed for ticks of immediately fed, semior completely gravid or completely digested blood ones. Our results indicate that due to very low specificity and time consuming, xenodiagnosis is not a useful method whereas PCR method has advantages for study the Borrelia prevalence in ticks

[Comparison of the effects of drying processes: vacuum oven, freeze, solar and microwave with traditional method on the qualitative characteristics of the Iranian saffron]

Saffron, the world's most expensive condiment, is one of Iran's most important non-petroleum exports. The drying method is determinant of saffron quality and price in the world market. So, the aim of this study was the comparison of the effects of saffron dehydration methods, like vacuum oven, freeze, microwave and solar drying with traditional method. During this experimental study and for production of saffron flowers, one of the farms in Gonabad was selected randomly and the flowers were picked and mixed thoroughly, together. The morphological specifications, percentage of yield of the stigmas, chemical test [moisture, ash, total nitrogen and, acid insoluble ash] were determined. Stigmas were dried. After dehydration the moisture content, total ash, acid insoluble ash, water soluble extract, total nitrogen, crude fiber, crocin, picrocrocin, saffranal and sensory specifications [color, texture and odor] were determined. The results of chemical analysis indicated that the solar dried, vacuum oven dried and microwave dried samples had preferable color [crocine] outcomes. However, from aromatic point of view [saffranal] traditional sample had significant preference [P<0.05]. The amount of the picrocrocin [taste compounds] in samples did not have significant differences within each other. The results of sensory analysis indicated that, color of dried samples by solar, freeze and microwave methods, soluble color of dried samples by solar, freeze and vacuum oven had significant differences [P<0.05] that other methods. The results of the microbiological test of all dried samples were in Iranian standard levels except the traditional and freeze dried samples. Although the samples of four methods of drying were of high quality compared to the traditional samples, the solar drying method can be reasonably applied in rural area as suggested method of drying