Subject(s)
Humans , Male , Female , Lung Neoplasms , Lymphocytes , Bhopal Accidental Release , Environmental ExposureABSTRACT
MicroRNAs (miRNAs) constitute an extensive class of non coding, gene regulating RNAs. Till date, there are no detailed reports of miRNAs in Zebra finch (Taeniopygia guttata). Here we adopt the method of homology search for the identification of miRNAs in T. guttata by using the pre-miRNAs of all known vertebrates. We report 221 miRNAs, of which 46 miRNAs are having 100% identity to pre miRNA and 208 miRNA to mature sequences. 104 sequences have mismatches at pre-miRNA level but have conserved mature miRNA. Thus the functions of mature sequences have been conserved during evolution of T. guttata genome. We also find that 157 miRNAs are closer to Gallus gallus, which is expected. Of which 117 sequences do not have 100% identity at pre-miRNA level. More importantly we find that 23 miRNAs come from platypus giving a remarkable clue to their relation with avian members. We also report conservation of 10 miRNA clusters. Five of these clusters are conserved with G. gallus, while the other 5 are altered with respect to gene orientation in the genome of T. guttata, and one among them is repositioned to chromosome 4a in T. guttata. These 5 altered gene orientations are unique to T. guttata genome and could have arisen due to divergence during evolution.