ABSTRACT
Background: although type-2 mellitus diabetes is the most common type of diabetes, it's main cause yet to be identified. Chemokines and their receptors are probable effective systems on diabetes. CCR5 is a chemokine receptor playing an important role in immune responses. Studies showed that the known delta32 mutation in CCR5 gene leads to disorder in the expression and function of this receptor. Hence, this project aimed to analyze the known delta32 mutation in CCR5 chemokine receptor
Materials and methods: blood samples were collected from 200 type 2 diabetic patients and 300 healthy adult controls on EDTA pre-coated tubes. DNA was extracted using commercial kit. DNA samples were analyzed for delta32 mutation by Gap-PCR in diabetic patients in compared to controls. The demographic information was collected through questionnaire
Results: our results showed that none of the diabetic patients displayed CCR5 delta32 mutation. While 2 out of 300 healthy controls had heterozigotic form of this mutation. Statistical analysis didn't show any significant difference between the two groups
Conclusion: several different studies analyzed the relation of this mutation with different types of diseases including diabetes. All studies failed to find a relation between this mutation and type 2diabetes. Since these studies were performed in different geographical points and races, we studied this mutation in Rafsanjani's population. Based on the results of our study it could be probably concluded that this mutation does not play a key role in the establishment of type 2 diabetes
ABSTRACT
Statement of problem: Sodium hypochlorite is used as an endodontic irrigant for different purposes. Since it is a strong oxidizing agent it might inhibit the resin polymerization
Purpose: The objective of this in vitro study was to compare the effect of NaOCl on sealing ability of total etch and self etch bonding at different time intervals after root canal therapy
Materials and Methods: The roof of the pulp chambers and roots [1-2 mm below furcation] of eighty human first molar teeth were cut and pulp tissues were completely removed. Teeth were divided in two groups [n=40], based on the type of used adhsive; total etch [Scotchbond Multi-Purpose plus] and self etch [Clearfil SE Bond]. Immediately, chamber pulp of ten teeth of each group were restored with resin composite [control groups]. Thirty teeth in each group were treated with 5% NaOCl for 5 minutes in the chamber pulp and restored with resin composite associated with each bonding agent [Z100, Clearfil Photo Core] in three intervals: immediately, one, and two weeks after irrigation with NaOCl. Fluid filtration method was used for evaluation of microleakage. Data were statistically analyzed using ANOVA and T-test [alpha =.05]
Results: Type of dentin adhesive and different time intervals after NaOCl application and restoration, did not show any significant effect on microleakage value [p>0.05]. The variable of using and not using of NaOCl showed significant difference on microleakage value [p<0.05]. NaOCl treated samples demonstrated significantly higher microleakage
Conclusion: Irrigation with NaOCl during root canal therapy has a negative effect on microleakage of resin composite restorations. Delayed time for restoration did not remove the negative effect of NaOCl