ABSTRACT
the plants with the less side effects and drug resistance have been used worldwide. In this study, we compared the inhibitory effects of nanocopper and essential oil of Melissa officinalis.La on Rcotiand S.mutans in In Vitro condition. for studying diameter of inhibitory zone, disk agar diffusion method was used. Then, antibacterial effects of these substances were evaluated by treating them for 24 hours in Nanocopper particles [concentration of 100 and 500 ppm] and essential oil [12.5% to 100%], and analyzed by ANOVA. twenty-four hours after treatment, nanocopper had no inhibitory effect on these bacteria. However, the diameter of inhibitory zone for E.coli and S.mutans was 31.30 +/- 0.13mm and 16.30 +/- 0.13mm, respectively. There was not any synergistic effect between different concentrations of this plant and 500pprn of nanocopper after 24 hours of treatment on E.coli. But for S. Mutans, the diameter of inhibitory zone for mixture of 50% and 25% essential oil and 500ppm concentration of nanocupper was increased significantly compared to only essential oil [p=0.001,p=0.01]. based on the findings, nanocopper particles and essential oil of Melissa officinatisL.a have not any synergic effects on E.coli, but with some concentrations of this plant the reverse is true on S.mutans
ABSTRACT
Flavonoids play an important role in non-enzymatic reaction against oxidative stress. These are polyphenolic compounds in tea structure that could be reacted with free radicals and neutralized them. In this study, we investigated the anti-oxidant impact of Camellia Sinesis on the liver of thioacetamide-injected male albino mice. In this study, 40 male mice were categorized in five groups of eight. The first group was control. The second and the third group received 100 mg/kg and 150mg/kg of thioacetamide, respectively. The fourth group received 100 mg/kg thioacetamide followed by black tea [5 gr/100] and the fifth one received 150mg/kg thioacetamide followed by black tea [5 gr/100]. Tioacetamide was given via intraperitoneal. After that, for 30 days, they were only fed on black tea [5 gr/100]. At the end, catalase [CAT] and glutathione peroxidase [GPx] activity were measured. Based on the results, catalase [CAT] and glutathione peroxidase [GPx] activity were significantly increased in the groups of Thioacetamide and black tea compared to those of only Thioacetamide groups [p<0.05]. The increase of these enzymes in tea groups shows the anti-oxidant effect of black tea that can be caused by Catechin
ABSTRACT
Chronic myeloid leukemia [CML] is a malignant clonal disorder of hematopoietic stem cells resulting in the increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. The research evaluated the cytotoxic effects of hydro-alcoholic extracts of Lepidium Sativum [Cress plant] shoots before and after flowering on K562 cell line as a model of CML. In this laboratory experimental study, the Lepidium Sativum shoots including stems and leaves of the plant before flowering and its shoots after flowering including stems, leaves and flowers were collected from Afoos city [Iran]. They were extracted using maceration [50% Ethanol 96% and 50% water] method. K562 cells were cultured. Then the cells were treated with different concentrations of the extract [12.5-100 micro g/ml] at different time intervals [24, 48 and 72 hour]. The Lepidium Sativum cytotoxicity was evaluated by the MTT test method before and after flowering against K562 leukemia cells. The absorption was measured using an ELISA plate reader at 540 nm wave length. Data were analyzed using SPSS15 software and one-way ANOVA test analysis as well as Tukey test; where P<0.05 was considered significant. Hydro-alcoholic extracts of Lepidium Sativum showed the most optimum cytotoxicity both before and after flowering with a dose of IC50=25 micro g/ml and 72 hour after treatment on K562 cell line. In other words, hydro-alcoholic extracts of Lepidium Sativum prepared before and after flowering exhibited a dose and time dependent cytotoxic effect on K562 cell line. Considering the cytotoxic effect of hydro-alcoholic extracts of Lepidium Sativum shoots before and after flowering on K562 cells, the plant can be considered as a potential candidate for further studies on CML treatment
ABSTRACT
particles are one of functional nanotechnology filed. These nanoparticles have antibacterial and antifungal characteritic. Combination therapy is one of new and specific method for therapy of diseases in medicine and pharmacology. Some plants are useful in growth inhibition of Aspergilus Niger. This study was done to evaluate the synergic effect of alcoholic eucalyptus and nanosilver on colony count of Aspergilus Niger. In this laboratory study PAN were cultured in PDA medium. Five experimental and one control group were designed for the comparison of synergic effect of alcoholic eucalyptus and nanosilver, nanosilver 50ppm, nanosilver 12.5ppm, eucalyptus ethanolic extract 100%, eucalyptus ethanolic extract 100% mixed with nanosilver 12.5ppm, eucalyptus ethanolic extract mixed with 50ppm, and double distilled water were given to group 1, 2, 3, 4, 5 and control group respectively. Morphological, diameter, and colony numbers in various culture media were compared subsequently. The repeated number of Aspergilus Niger were 24, and they were chosen randomly. Data were analyzed using SPSS-15 and ANOVA test. The Aspergilus niger colony were reduced 8 days following treatment from 200 colony in control group to 90, 75, 55 and 43 in nanosilver group in 12.5 ppm, nanosilver group in 50 ppm, nanosilver group in 50 ppm mixed with eucalyptus ethanolic extract and finally eucalyptus ethanolic extract group, respectivley [P<0.05]. The colony count 24 day following treatment were reduced from 200 colony in control group to 42, 14 and 2 in eucalyptus ethanolic extract group, nanosilver group in 12.5 ppm mixed with eucalyptus ethanolic extract and finally nanosilver group in 50 ppm mixed with eucalyptus ethanolic extract, respectivley [P<0.05]. This study showed that nanosilver with 50 ppm concentration mixed with eucalyptus ethanolic extract have synergic effect on reducing the Aspergilus niger growth rate
ABSTRACT
In recent years, the resistance of opportunistic fungal strains to commercially available antifungal agents has been increased. The serious side effects of these compounds on mammalian cells forced the researchers to search for new antifungal substances. Thus we decided to investigate the antifungal properties of silver nanoparticles against Aspergillus fumigatus, To investigate the antifungal effect of the round silver nanoparticles with lOnm diameter against Aspergillus fumigatus, the diameter of colonies after 8 days as well as the number of colonies in different days was determined, using direct drop test. After that, to measure the Minimum Inhibitory Concentration [MIC] and Minimum Fungicide Concentration [MFC] values of silver nanoparticles and fluconazole, Micro Dilution Broth method was performed. At the end, the MIC and MFC values of silver nanoparticles were compared to MIC and MFC of fluconazole. The results obtained from direct drop test confirm that the silver nanoparticles can decrease the diameter of fungal colonies in dose dependent manner. The data of silver nanoparticles on the number of colonies in different days shows that the number of colonies increases up to sixth day and then becomes fixed. Based on the results of Micro Dilution Broth method, the MIC and the MFC values of this component are 31.25 and 62.5ppm, respectively. The present study confirms that silver nanoparticles with l0nm diameter have antifungal activity against Aspergillus fumigatus
ABSTRACT
Nanosilver particles are one of the functional nanotechnology filed. These nanoparticles have antibacterial characteritics. Combination therapy is one of new and specific therapeutic regiment in medicine. Eucalyptus plant is useful in growth inhibition of bacteria. This study was done to evaluate the inhibitory effect of alcoholic eucalyptus extract with nanosilver on E.coli growth. In this laboratory study, E.coli was cultured in nutrient agar medium. Primarily 50 microl of bacteria was inocolulated in each plate, antibiograms disc contaminated with 3.1, 6.25, 12.5, 25 and 50 ppm concentration of nanosilver accompanied with 100% of etanolic extract of eucalyptus were placed in each plate subsequently. Diameter of inhibitory zone were evaluated following 24, 48, 72 hrs in 6[th] and 8[th] days. Data were analyzed using SPSS-15, ANOVA and Tukey tests. After six days, inhibitory growth zone diameter of E.coli was 0.5 mm. This diameter in experimental group with 3.1, 6.25, 12.5, 25 and 50 ppm of nanosilver in combination with etanolic extract of eucalyptus were 0.55, 0.58, 0.82, 0.83 and 1.02 mm. Inhibitory growth zone in group of 12.5, 25 and 50 ppm of nanosilver was significant in compared to the control [P<0.05]. The most proper time of inhibitory effect on E.coli growth is six day after treatment in combination of 50 ppm nanosilver particles with extract of eucalyptus
ABSTRACT
Nanoparticles are very small pieces of material that have different applications in medical fields. Nanosilver technology is a functional branch in nanotechnology. Different studies have proved antimicrobial effects and useful functions of nanosilver in biotechnology field and its specific inhibitory effects on microbes. As no exact report about antibacterial effects of these nanoparticles has been made, this study was conducted to investigate silver inhibitory effects on E.coli. Nanosilver particles at concentration of 100, 200, 300, 400 and 500ppm were inseminated on blank anti biogram discs and were placed on cultivated nutrient agar environment by 0.5 Mac Farland`s standard. Then, inhibition zone diameter was measured in the first, second, and sixth day. T-test was used to compare the average inhibition zone in control and treatment groups and the value p<0.05 was considered as statistically significant. In the first day after the treatment of nanosilver particles at a concentration of 400ppm, the average inhibition zone diameter was 2.30 +/- 0.43mm in E.coli that has been increased significantly in comparison with control culture [p=0.01]. In the second day after treatment at a concentration of 400ppm, the average disc diameter was 2.48 +/- 0.39mm which shows that these values have been increased significantly in comparison with control groups [p=0.01], but they were not significantly different from the first day. Since Nonotechnology has various applications in different fields, expanding these results could be helpful in biomedical therapeutic of bacteria diseases. In conclusion, our results may provide this important insight that nanoparticles could replace many antibiotics without many side effects
Subject(s)
Silver , NanoparticlesABSTRACT
Gene therapy is a new and specific method for therapy of leukemia. Antitelomerase agents can be used in cancer therapy. Telomeres are specialized heterochromatin structures that act as protective caps at the ends of linear eukaryotic chromosomes. Human telomeres consist of tandem repeats of the hexanucleotide sequence TTAGGG in double strand. Telomerase is detected in the majority of cancers, but not in normal tissue. Telomere maintenance in 80-95% of tumor cells is achieved by telomerase. The aim of this research was to assess the inhibitory effects of nano-oligonucleotides as a new nano technology method in gene therapy of leukemia in vitro. In this study, we employed P5N3 phosphoramidate oliogoadenylate synthetic, called sense, antisense and scramble. In this research, oligomers with.5 micromolr did not have any effect on telomerase phosphoramidate P5activity. But telomerase activity significantly decreased with N3 hTR sense and antisense after 3 days with 1 to 3 micro molar in comparison with control groups. One of the reasons for inhibition of telomerase via the aforementioned antisense is to link them with RNA telomere named hTR and hence prevent this enzyme from functioning. By doing this, in fact, hTR is dissolved. This method in vitro can be used in human leukemia