ABSTRACT
Transfusion transmitted HIV is an important issue in every transfusion center. Despite recent improvements in laboratory screening techniques and formulation of rigid standards for blood safety, the risk of HIVcontaminated blood products remains as a potential problem. This study was conducted to determine the knowledge, attitude and practice regarding blood donation among voluntary blood donors about HIV/AIDS. This is a cross-sectional study in which data were collected through questionnaires.We studied knowledge, attitude and behavior of 440 voluntary blood donors about HIV/AIDS in Sistan and Balouchestan province. Voluntary blood donors' knowledge and attitude were evaluated and their correlation with different variables were compared. The data were analyzed with Chi- Square, ANOVA, Kruscal Wallis and Spearmans rho using SPSS 14. Our results showed that the knowledge levels of 55% of voluntary blood donors about HIV/AIDS were good, 29.5% intermediate, and 15.5% poor. Seventy point one percent of population in the study had positive attitude, 56.9% had neutral attitude and 25.9% negative attitude about HIV/AIDS. The correlation between sex, marriage status, educational status, job, type of donation [voluntary or familial], times of donation [first, repeated and regular repeated] with knowledge and attitude was statistically significant. Relatively high level of knowledge about HIV/AIDS especially in regular repeated donors can be due to acquired information during consultation sessions. Therefore, repeated and regular repeated donors can help improve the blood safety.These findings also highlight the importance of more accurate consultation and the provision of necessary training on HIV/AIDS to voluntary blood donors
Subject(s)
Humans , Blood Donors , HIV , Acquired Immunodeficiency Syndrome , Cross-Sectional Studies , Surveys and QuestionnairesABSTRACT
In most cases, after the surgical procedures were completed, the area was covered with a surgical pack. Dentin hypersensitivity is one of the common problems after periodontal surgeries. Calcium Hydroxide is an inexpensive and available material used for desensitizing. The purpose of this study was to evaluate the cytotoxicity of mixture of calcium hydroxide and periodontal dressing on L929 fibroblasts. In this study Rat fibroblasts were used. For preparing extracts, we added 0, 1, 5 and 10 mg of calcium hydroxide to 1 gr of periodontal dressing. Then, they were placed in autoclave followed by 5[cc] of basal media [DMEM].A control group consisting of L929 fibroblasts plus basal media was also considered. After 24, 48 and 72 hours incubation, we examined the numbers [quantity] as well as the morphology of the cells [quality]. For quantitative evaluation [MTT assay] after adding Tetrazolium salt to cells, we read the optical density of each plate using ELISA reader. The data were analyzed statistically using chi-square and Kruskal wallis test. All of the plates had the same quality but the cells in the control group showed more proliferation. All of the plates had plenty of vital and normal fibroblasts but in comparison with the control group the cells had developed less proliferation. Statistical test analysis of the data showed a significant difference between the optical density of the experimental plates and the control group indicating that the number of vital cells in control group was significantly greater than the test groups. Because the number of active vital cells in the plates with periodontal dressing was equal to other plates but less than control group, it can be concluded that the cytotoxic effects in the different plates were related to periodontal dressing, not Calcium hydroxide