ABSTRACT
The food handlers control is one of the major measures of the National program against intestinal parasitoses in Tunisia. The collected data allow regular updates of the prevalence and profile of the involved parasitic species and note possible evolutions in time. From 1998 to 2008, 8502 stool samples of food handlers working in Tunis area were examined in the laboratory of Parasitology, Pasteur Institute of Tunis. The mean prevalence of intestinal parasites was 13.5% [1152 positive samples]. The protozoa were the most detected parasites [93% of the species observed]. Among the pathogenic or potentially pathogenic parasites, Dientamoeba fragilis [15.5%] and Giardia intestinalis [7.6%] were the most frequently encountered. Entamoeba [E.] histolytica/dispar was diagnosed in 41 subjects [a prevalence of 0.5%]. Hymenolepis nana was the most frequent helminth [4.5% of the whole parasites]. Therapeutic failures were noticed in 43.8% of infection by this cestode because of the lack of the appropr iate antihelminthic. The identification using PCR of cysts and/or vegetative forms of E. histolytica/dispar revealed the exclusive presence of E. dispar