[Effects of hydroethanolic extract of kelussia odoratissima Mozaff. leaf on total antioxidant capacity and BMP7 gene expression in rat white adipose tissue]

Background: Obesity is one of the problems of major concern to today's society. Weight loss has been reported for Kelussia odoratissima Mozaff

Objective: Aim of this research is to examine the effect of this plant extract on plasma total antioxidant capacity and Bone Morphogenetic Protein 7 [BMP7] gene expressions in white adipose tissue [WAT]

Methods: Eighty male wistar rats were divided in two prevention [A] and treatment [B] groups and every group were divided to four subgroups. The A for prevention from obesity and B were used for reducing of obesity. WAT was obtained after the study for BMP7 gene expression [with using Real time PCR]. Liver sample for catalase activity, blood for measuring of total antioxidant capacity and paraoxonase 1 activity were prepared

Results: Weight loss and BMP7 gene expression was seen only in subgroup that receiving K. odoratissima hydroethanolic extract in the A group. Contrary to expectation, K. odoratissima extract was reduced the total antioxidant capacity in both groups, reduced level of serum paraoxonase 1 activity and increased liver catalase [p value = 0.002] in comparing to the subgroup that received high fat diet [p value = 0.011]

Conclusion: K. odoratissima hydroethanolic extract has an effective role in prevention of weight gain and enhanced liver catalase activity. Increasing in BMP7 gene expression probably causes alteration of WAT to brown adipose tissue [BAT]. According to this study, consumption of extract can reduce serum total antioxidant capacity and is likely to exacerbate oxidative stress

Gene cloning of Iranian Leishmania major mannose-1-phosphate guanyltransferase

Leishmania is an obligatory intracellular protozoan parasite, which infects human beings when infected sand fly vector takes a blood meal. Most efforts are towards designing an effective vaccine to prevent leishmaniasis. In this way, development of candidate antigen for vaccine has special important. In this study, we cloned mannose-1-phosphate guanyltransferase gene of Iranian L .major in pET32a expression vector. Primers based on L. major mannose-1-phosphate guanyltransferase sequence gene was designed and synthesized. DNA of Leishmania promastigotes was extracted and PCR reaction was done. PCR product was cloned into pTZ57R and sub cloned into pET32a expression vector. Recombinant plasmid containing 1140 bp as L. major mannose-1-phosphate guanyltransferase gene was extracted and confirmed by restriction analysis. PCR product was sequenced and deposited to GenBank. There were some differences in amino acid sequences between Iranian L. major mannose-1-phosphate guanyltransferase and others previously accepted in GenBank. We amplified and cloned Iranian L. major mannose-1-phosphate guanyltransferase successfully