Cytomorphologic and immunocytochemical study of touch imprints of malignant Non-Hodgkin's Lymphomas [NHL]

The present study reports on experience with the application of lymphocyte markers [4KB5, UCHL1 and MAC 387] to lymph node imprints in NHL. The cytologic and immunophenotypic classification of the imprints were compared with those of the corresponding tissue sections. Among the 50 cases studied 43 had a B-cell phenotype, 3 had a T-cell phenotype, while 4 cases defied any staining. Using immunohistochemistry, it was not only possible to identify the phenotype of the cells but to decide whether the lymphoid population was polyclonal or monoclonal as well. Demonstration of monoclonal light chain restriction in B-cell lymphoma made it possible to confirm the neoplastic nature of the imprint. The present work emphasized the value of imprint material as good source of tissue for immunologic study, where immunoreactivity to MoAbs was retained by storage of the imprints at -70C. The advantages of this technique include enhanced cellular detail, clear staining reaction with reduced artefactual distortion in addition to a more rapid methodology

immunohistochemical study on the histogenesis of granular cell tumor

The presence and distribution of S-100 protein, desmin and alpha-1- antichymotrypsin were investigated in ten cases of granular cell tumor using the peroxidase-antiperoxidase method. The granular cells of all cases were negatively stained with anti-desmin and anti-alpha-1-antichymotrypsin antisera. On the other hand, they were positively stained with anti-S-100 protein antiserum. These results supported the concept of the neurogenic origin of the granular cell tumor

Demonstration of alpha-1 antichymotrypsin in tumours of supposed bibroblastichistocytic origin using the immunoperoxidase technique

Using the immunoperoxidase [PAP] technique, 28 cases of fibrohistiocytic tumors were stained for alpha-1-antichymotrypsin [A1ACT], a histiocytic marker, and S-100 protein, a marker for Schwann cells. A1ACT was demonstrated in 12 of 13 cases of malignant fibrous histiocytomas [MFH], in 6 of 9 cases of dermatofibrosarcoma protuberans [DFP] and in 4 of 6 cases of dermatofibroma [DF]. None of these tumors stained positively for S-100 protein. A1ACT is found to be a useful and reliable marker for malignant fibrous histiocytoma. The presence of alpha-1 antichymotrypsin and the absence of S-100 protein in DFP and DF support the concept that these neoplasms are parts of the spectrum of fibroblastic-histiocytic tumors and excludes their neurogenic origin