ABSTRACT
An in-vitro study was designed to focus on effects of verapamil on the metabolic potential of cardiac slices after reversible ischemia in rats. The study included two main groups: Group A, non ischemia/reperfusion group and group B, ischemia/reperfusion group. Each was subdivided into two subgroups [a and b], each subgroup included ten experiments. Ischemic cardiac slices were obtained from rats subjected to 10 min hemorrhage to induce reversible global ischemia. The results revealed that there was an enhancement in the release of free fatty acids, and lactate and in glucose uptake in group Ba as compared with group Aa. These metabolic alternations produced by ischemic cardiac slices were revealed by verapamil addition but in group Ab, verapamil did not alter the release of FFA and lactate from non- ischemic cardiac slices, whereas it inhibited glucose uptake from these slices. The improvement of the metabolic alternation of ischemic myocardium indicates that verapamil may be of importance in reducing the extent and severity of acute myocardial ischemic injury in acute hemorrhage
Subject(s)
Calcium Channel Blockers , Myocardial Reperfusion , Wounds and InjuriesABSTRACT
The use of verapamil as cardioprotective agents for management of patients with acute ischemic/reperfused heart is based on the assumption that increased intracellular Ca++ level is a key factor in causing cell death. The in vitro study was designed to focus on effects of verapamil on the metabolic potential of cardiac slices after reversible ischemia in rats. The study consisted of two main groups: group A [non ischemia/reperfusion group] and group B [ischemia/reperfusion group]. Each was subdivided into two subgroups [a and b]. Each subgroup included 10 experiments. Group Aa was the control group, Group Ab was verapamil added group, Group Ba was ischemic group without verapamil, Group Bb was verapamil added group. Ischemic cardiac slices were obtained from rats subjected to 10 min. hemorrhage to induce reversible global ischemia. Both nonischemic and ischemic cardiac slices were placed in well oxygenated Krebs-Ringer phosphate buffer contained 150 mg% glucose and 3 gm% bovine albumin and included in Dubnoff shaking water bath for 60 min. at 37C. The results revealed that there was an enhancement in release of free fatty acids; [FFA, 240%] and lactate [163%] and in glucose uptake [160%] in group Ba as compared with group Aa. These metabolic alternations produced by ischemic cardiac slices were reversed by verapamil addition [200 ul%] but in group Ab verapamil did not alter the release of FFA and lactate from non-ischemic cardiac slices, whereas it inhibited glucose uptake from these slices by 67%. The improvement of the metabolic alternation of ischemic myocardium indicated that verapamil may be of importance in reducing the extent and severity of acute myocardial ischemic injury in acute hemorrhage