ABSTRACT
Tanshinone Ⅱ_A( Tan Ⅱ_A),the liposoluble constituents of Salvia miltiorrhiza,can not only ameliorate the lipidic metabolism and decrease the concentration of lipid peroxidation,but also resist oxidation damage,scavenge free radicals and control inflammation,with a protective effect on prognosis after liver function impairment. Therefore,the studies on the exact mechanism of Tan Ⅱ_A in protecting the liver can provide important theoretical and experimental basis for the prevention and treatment effect of Tan Ⅱ_A for liver injury. In the present study,the protective effects and mechanism of Tan Ⅱ_A on 4-hydroxynonenal( 4-HNE)-induced liver injury were investigated in vitro. Normal liver tissues NCTC 1469 cells were used to induce hepatocytes oxidative damages by 4-HNE treatment. The protective effect of Tan Ⅱ_A on hepatocytes oxidative damages was detected by release amount of lactate dehydrogenase( LDH) analysis and hoechst staining. The protein expression changes of peroxisome proliferator-activated receptor α( PPARα) and peroxisome proliferator response element( PPRE) were analyzed by Western blot analysis in NCTC 1469 cells before and after Tan Ⅱ_A treatment. The gene expression changes of fatty aldehyde dehydrogenase( FALDH) were analyzed by Real-time polymerase chain reaction( PCR) analysis. The results showed that 4-HNE increased the release amount of LDH,lowered the cell viability of NCTC 1469 cells,and Tan Ⅱ_A reversed 4-HNE-induced hepatocyte damage. Western blot analysis and RT-PCR analysis results showed that 4-HNE decreased the expression of PPARα and FALDH and increased the expression of 4-HNE. However,the expression of PPARα and FALDH were increased significantly and the expression of 4-HNE was decreased obviously after Tan Ⅱ_A treatment. This study confirmed that the curative effect of Tan Ⅱ_A was obvious on hepatocytes damage,and the mechanism may be associated with activating PPARα and FALDH expression as well as scavenging 4-HNE.
Subject(s)
Animals , Mice , Aldehyde Oxidoreductases , Metabolism , Aldehydes , Cell Line , Abietanes , Pharmacology , Hepatocytes , Lipid Peroxidation , Oxidative Stress , PPAR alpha , MetabolismABSTRACT
Objective To explore the risk factor of chronic disease-related behaviors such as smoking, drinking, exercising and eating habit among residents in Liubei District, Liuzhou City. Methods 2 487 adult residents in Liubei District were sampled by multistage sampling method and surveyed on their way of life and behavior risk factors. The content of questionnaire involved familial population information, past medical history, way of life and eating habit. EpiData 3.1 was applied to install database, SPSS 17.0 was used for data analysis, multivariate Logistic regression was used to analyze the risk factor of chronic disease-related behaviors. Results The rates of hypertension, diabetes, stroke, chronic obstructive pulmonary disease (COPD) were 13.63%，4.22%，3.62%,0.36% and 6.84%, respectively. The rate of smoking, drinking, doing physical exercise and eat breakfast regularly were 22.92%, 5.43%，52.87% and 93.07%. Multivariate Logistic regression analysis showed that older and smoking were risk factors of chronic disease, while physical exercise was protective factor of chronic disease. Conclusions Intervening measure taken in chronic disease demonstration zone were effective in the past 5 years， and health education on behavioral intervention should be carried on continuous to reduce incidence of chronic disease.
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Objective To study the clinical effect of Periocline ointment as the auxiliary treatment of chronic periodontitis. Methods 100 cases of chronic periodontitis in our hospital were selected as the subjects (from May 2016 to June 2017). The patients in the experimental group and the control group underwent periodontal examination, and the baseline was recorded. The control group was treated with routine treatment, the patients treated on the basis of analysis of Periocline ointment in the treatment of two groups of patients treated for 4 weeks. Results After treatment, the patients in the experimental group for attachment level (4.84±0.74) mm, probing depth (3.45±0.47) mm, bleeding index for (2.43±0.52) was significantly better than the control group related clinical indicators of periodontal probing depth and attachment level, with statistical significance (P<0.05). The number of effective cases in the experimental group was 47 cases (94%), significantly higher than the control group, the number of effective treatment in 44 cases (88%), the difference was statistically significant (P<0.05). Conclusion Periocline ointment as the auxiliary treatment of chronic periodontitis can improve the treatment efficiency, improve clinical indexes of patients, is worthy of clinical application.
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Objective To study the clinical effect of Periocline ointment as the auxiliary treatment of chronic periodontitis. Methods 100 cases of chronic periodontitis in our hospital were selected as the subjects (from May 2016 to June 2017). The patients in the experimental group and the control group underwent periodontal examination, and the baseline was recorded. The control group was treated with routine treatment, the patients treated on the basis of analysis of Periocline ointment in the treatment of two groups of patients treated for 4 weeks. Results After treatment, the patients in the experimental group for attachment level (4.84±0.74) mm, probing depth (3.45±0.47) mm, bleeding index for (2.43±0.52) was significantly better than the control group related clinical indicators of periodontal probing depth and attachment level, with statistical significance (P<0.05). The number of effective cases in the experimental group was 47 cases (94%), significantly higher than the control group, the number of effective treatment in 44 cases (88%), the difference was statistically significant (P<0.05). Conclusion Periocline ointment as the auxiliary treatment of chronic periodontitis can improve the treatment efficiency, improve clinical indexes of patients, is worthy of clinical application.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the association between physical activity (PA) and the incidence of metabolic syndrome (MS) in Chinese adults.</p><p><b>METHODS</b>Data on PA and other variables were obtained at the baseline examination of China Multi-center study of Cardiovascular Epidemiology in 1998 and of International Collaborative study of Cardiovascular Disease in Asia(InterASIA) during 2000 - 2001. Follow-up study was conducted in 2007 - 2008. A total of 11 512 Chinese adults aged 35 - 74 years (5563 men and 5949 women) were included in the final data analysis. Information on demographics, PA, smoking and alcohol consumption were obtained and components of MS were examined. Participants were divided into four groups according to quartile of total metabolic equivalent (MET) values per day. In addition, subjects were grouped into the following categories according to occupational PA: inactive, light, moderate and vigorous. Binary logistic model was used to examine the association between PA and the incidence of MS.</p><p><b>RESULTS</b>A total of 2527 cases with MS were documented during an average following up of 8.1 years. The annual incidence rate of MS was 2.71% (2527/93 178.68). After multivariate logistic regression analysis, compared with participants with total PA volume < 32.0 MET×h×d(-1) (annual incidence rate was 3.19% (697/21 830.74)), the RR (95%CI) value of participants with total PA volume during 32.00 - 37.85, 37.86 - 52.29, and ≥ 52.30 MET×h×d(-1) was 1.05(0.92 - 1.19), 0.98(0.86 - 1.12), and 0.68(0.59 - 0.80), respectively (χ(2)trend = 34.23, P < 0.05), with corresponding annual incidence rates of 2.82% (690/24 504.25), 2.73% (661/24 179.36) and 2.11% (479/22 664.33). In addition, compared to inactive occupational PA (annual incidence rate was 2.76% (402/14 588.33)), the corresponding RR (95%CI) value was 0.80 (0.69 - 0.92), 0.70 (0.59 - 0.82), and 0.54 (0.45 - 0.65) (χ(2)trend = 42.34, P < 0.05), and the annual incidence rates were 2.86% (648/22 663.41), 2.40% (455/18 956.14) and 1.89% (344/18 173.86) in participants with light, moderate and vigorous occupational PA, respectively.</p><p><b>CONCLUSION</b>Both increased total PA volume and occupational PA intensity are significantly associated with decreased risk of incidence of MS.</p>