Potential value of monoclonal antibody [B18] against carcinoembryonic antigen in diagnosis of adenocarcinoma of the bladder

The present study describes the tissue reactivity of bladder adenocarcinoma associated with bilharzial cystitis against B18 monoclonal antibody. This antibody was generated against carcinoembryonic antigen producing human adenocarcinoma. The specificity of this antibody was tested by immunoperoxidase technique on paraffin sections from bladder adenocarcinoma, normal and glandular metaplastic urothelium. Adenocarcinoma of the bladder were positive the B18 MAB against CEA did not stain normal and glandular metaplastic urothelium. So, the antibody may be a useful immunohistochemical probe as tumor marker of bladder adenocarcinoma co-existing with cystitis glandularis, especially in superficial preoperative bladder biopsy and could confirm the malignant nature of cellular atypia detected in cysitis glandularis

Involucrin expression in bladder carcinoma: an immunohistochemical study

Involucrin is a protein component of the cross-linked envelope synthesized by maturing cells of human stratified squamous epithelia immediately beneath the plasma membrane. The reactivity of this antibody was tested by immunoperoxidase technique on paraffin sections from bladder carcinoma of different morphological and histological grades in order to evaluate the usefulness of involucrin as a tumor marker in bladder neoplasms. Although involucrin is absent from most of transitional epithelium adjacent to bladder carcinoma, it is expressed in squamous metaplasia, but in adjoining areas of carcinoma in situ the staining pattern is altered. Involucrin was present in all cases of grade I squamous cell carcinoma most marked in larger neoplastic cells in the center of squamous cell nest. Focal staining was observed in some adenocarcinoma and invasive high grade transitional cell carcinoma. These cases may represent adenosquamous variant or squamous metaplasia in transitional carcinoma, isolated involucrin positive cells were present in few cases of anaplastic carcinoma. Immunohistochemical staining for involucrin is a sensitive marker for squamous differentiation in bladder carcinoma. Staining pattern in intraepthelial carcinoma may identify abnormal or premature involucrin formation and hence cellular differentaition, areas of special promise include early diagnosis of intraepithelial changes