Biochemical study on normal and leukemic Egyptian children

Leukemia is the most common malignancy of childhood [33%] where Acute Lymphoblastic Leukemia [ALL] is the most common type in children. is to study the effect of ALL on complete blood count [CBC] and on some important biochemical parameters as total protein, albomin, globulin, uric acid, lactate dehydrogenase [LDH], erythrocyte superoxide dismutase [eryth. SOD] and erythrocyte glucose-6 -phosphate dehydrogenase[eryth. G-6-PD]. Also to measure total DNA and RNA content in all the samples. Moreover to carry out a comparative study of these parameters before and after treatment in contrast to control group. This study included forty newly diagnosed children with [ALL] in National Cancer Institute [NCI], Cairo University. Blood samples on admission [Gr. IIA] and after 4 weeks of chemotherapeutic treatment [Gr. IIB] were analyzed. Four cases died 2 weeks from diagnosis, one case discontinued, and one case failed to remission induction. CBC revealed a significant remarkable decrease in the hemoglobin content, RBCs and platelet count in [Gr. IIA] and these parameters were improved in [Gr. IIB]. A significantly marked elevation in WBCs and blast cells were recorded in [Gr. IIA]. On remission they returned to their normal level in [Gr. IIB]. Total DNA and RNA contents revealed a significant remarkable elevation in [Gr. IIA], while after 4 weeks of treatment they returned to normal level [Gr. IIB]. G-6-PDH showed significant increase in [Gr. IIA] while SOD activity showed high significant decline in [Gr. IIA]. After treatment both G- 6-PD and SOD showed significant improvement [Gr. IIB]. One month of chemotherapeutic treatment is not enough to achieve complete hematological remission for Egyptian [ALL] children in contrast to European reports. Biochemical parameters returned to their normal level, yet G-6-PD and SOD activities were improved largely. Also measurement of G-6-PD and SOD activities and DNA and RNA contents are useful markers to follow up ALL children after chemotherapeutic treatment

Nutritional and physiological studies on onion allium cepal: 1 Effect on blood constituents

The results indicated that onion with 1% and 2% additions per kg diet caused a significant decrease in the number of red blood cells, packed cell volume and haemoglobin content of the blood. On the other hand it increased the number of while blood cells. Blood smears were prepared from control and treated rats. They were stained with new methylene blue and modified wright's stains for detection of Heinz bodies and reticulocytes. No evidence could be noticed for the presence of Heinz bodies or reticulocytes in the blood of rats as a result of adding onion to its feed. The glucose level in the blood of treated animals was significantly lower than its level in the control ones. Total protein was increased in the serum of the treated rats. The cholesterol content in the serum of rats was highly significant increased in the fourth group in which 3% onion per kg diet was added to the feed of rats when compared with cholesterol level in the serum of control animals