Factors affecting the maximum yield of non protein nitrogen from skeletal muscles

Attempt was made to study the factors that affect maximum yield of nonprotein nitrogen from various skeletal muscles. The results showed that sonification was the most effective method out of the various extraction procedures used for the extraction of NPN, while the use of tungstic acid as protein precipitant was most suitable. The pH studies showed that pH 2 gave the optimal yield of NPN. Results of the coloured sample dilutions showed exponential relationship between optical densities and the concentrations of NPN used. The results are discussed in terms of the factors affecting maximum yield of NPN along with the significance of the term half optical density dilution

Electrocardiographic studies in the patients of Duchenne muscular dystrophy

Electrocardiographic [ECG] data from twenty Duchenne dystrophic patients aged 6 - 18 years were examined and compared with normal standards to explore the characteristic ECG abnormalities in these patients. Pathological electrocardiographic patterns were seen in 13 cases whereas 7 electrocardiograms were found normal. Amongst these 13 cases, the ECG of seven patients showed right ventricular hypertrophy [RVH] with strain. The ECG changes of asymmetric septal hypertrophy [ASH] were also observed in these patients. Four patients showed a pattern indicating combined ventricular hypertrophy [CVH] and one had typical low voltage changes

Osmotic fragility of erythrocytes in Duchenne muscular dystrophy

Osmotic fragility of erythrocytes was studied in ten Duchenne dystrophic patients and eight healthy controls. Lysis strated in Duchenne erythrocytes at a concentration of 6.5 g/1 NaC1 and in the control erythrocytes at a concentration of 5.5 g/1 NaC1. At all concentrations of sodium chloride [from 6.5 g/1 to 4.5 g/1] significantly greater lysis was observed in the erythrocytes of dystrophic patients. The median corpuscular fragility [MCF] was also significantly greater in Duchenne patients as compared to their controls [P< 0.001]. The increased osmotic fragility in Duchenne erythrocytes provides an evidence in support of the probable generalized membrane defect that manifest the disease

critical assessment of the experimental techniques for cardiac performance

Classical haemodynamics has been notably successful in defining the normal physiology of the Cardiovascular system, but failed to provide a clear definition and early diagnosis of a functional impairment of the myocardium, this failure is possibly attributable to inadequacy of the monitored variables of the conventional haemodynamics studies. In this article, two common parameters of cardiodynamics, viz., cardiac output and first derivatives of the ventricular pressure [dP/dt], are discussed in order to find properties of cardiac muscle which could help in distinguishing alterations of ventricular performance

Techniques used for the assessment of myocardial contractile force

Although several cardiovascular parameters are available to define the normal physiology as well as the pathophysiology of cardiovascular system yet a clear definition and early diagnosis of a functional impairment of the myocardium is lacking. The reason for this lacking may be the inadequacy of the monitoring of these variable parameters. One of the parameters among these is myocardial contractile force. This important variable has been evaluated in both experimental as well as clinical conditions in this present paper