Genistein diminished CIZ1 expression in daudi lymphoid cells.

Cip-interacting zinc finger protein 1 (Ciz1), stimulates DNA replication and has been implicated in tumorigenesis of breast cancer cells. In order to investigate the possibility of using medicinal isoflavone agaist breast cancer, we studied whether some isoflavones could affect the expression of the Ciz1. The in vitro effect of isoflavone treatment on the reduction of Ciz1 expression was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Western blotting also confirmed the down-regulation of the protein at dose dependent manner of the genistein treatment in Daudi lymphoid cell line cells.