occurrence of nocardia species among patients with pulmonary infection

The aim is to determine the frequency of Nocardia spp. among tuberculous patients in Khartoum state. Prospective study. Abu-Anga Teaching Hospital, El-Shaab Teaching Hospital and the Tuberculosis Reference Laboratory at the National Health Laboratory, Khartoum, Sudan. Three hundred and twenty-nine patients were included in this study during the period from October 2004 to January 2006. The patients were examined for the presence of acid-fast bacilli. Thin bacterial smears were prepared and stained by Ziehl Neelsen [ZN] stain. Two tubes of the Lowenstein-Jensen [LJ] medium were inoculated with 20 ul of the neutralized sputum sample that was obtained from the digestion and decontamination. One of the two tubes contained glycerol while the other tube contained pyruvic acid to isolate M. bovis species if encountered. All cultures were incubated at 37°C for 8 weeks. The growth was monitored up to the 8[th] week. Phenotypic characterization was performed by using different biochemical tests. Ten isolates showed rapid growth pattern within 2-3 days after inoculation. Further conventional methods suggested that all these isolates were belonging to the family Nocardiaceae. Nocardia spp revealed considerable occurrence among patients with pulmonary infections [3.3%]. This finding suggested that pulmonary nocardiosis might occur in patients who suffer from chronic lung disease in Sudan

Evaluation of a PCR-amplified IS6110 insertion element in the rapid diagnosis of pulmonary tuberculosis in comparison to microscopic methods in Sudan

The aim of this study was to evaluate the sensitivity and specificity of a polymerase chain reaction [PCR]-based method [IS6110 insertion site] in the diagnosis of pulmonary tuberculosis in sputum samples in comparison to smears by using culture on Loewenstein-Jensen medium as a standard. The study was conducted during the period 1999 through to 2000, at Khartoum Teaching Hospital, Sudan, on 200 sputum samples. The samples were collected from patients suspected of having pulmonary tuberculosis, were examined using a PCR amplified IS6110 insertion element in comparison to Ziehl-Neelsen stained smears in terms of sensitivity and specificity. Culture on Loewenstein-Jensen medium was used as the standard to control the 2 tests. Microscope sensitivity was found to be 65.4% and the specificity was 90.5%, whereas sensitivity of the IS6110 was 88.5% and specificity was 98.6%. The study concluded that though IS6110 sensitivity was 13.1% higher than smear method, it provided a significant improvement in specificity for the diagnosis of pulmonary tuberculosis. Improvement is still needed to increase the sensitivity of the IS6110 methods by decreasing the number of the false negative samples before its use can be at routine levels