protective effect of olive leaves extract or bone marrow mesenchymal stem cells on skin tissue damage induced in gamma irradiated rats

Aim of the work: This study aimed to examine the histological and histochemical changes in the skin tissue of male rats after exposure to gamma radiation and the possible therapeutic effect of either olive leaf extract or bone marrow mesenchymal stem cells

Material and methods: The present study was applied on forty adult male albino rats [Sprague Dawely strain]. The rats were categorized equally into five groups [1-C group: control rats; 2- O group: rats treated with plant leaves extract [15 mg/kg b. wt. /daily]; 3-R group: rats exposed to a single dose of gamma-radiation [3 Gy]; 4-RO group: rats of this group treated with olive leaf extract 15 mg /kg b.wt./daily one week prior to irradiation and one week post irradiation; 5- RS group: rats of this group were irradiated with 3Gy then treated with bone marrow mesenchymal stem cells [3×106 cells / ml suspension] through the caudal vein about 5 hours post radiation exposure. Histopathological and some common histochemical changes were studied

Results: rats exposed to gamma radiation showed several histological and histochemical changes, these changes were improved by using either olive extract or bone marrow mesenchymal stem cells. The bone marrow mesenchymal stem cells [BMSCs] showed more obvious curative effect than olive leaf extract

Conclusion: the present work showed that both olive leaf extract and bone marrow mesenchymal stem cells have skin tissue radiotherapeutic effects against gamma radiation in male albino rats

possible protective role of bone marrow transplantation against alternations induced by gamma radiations on fetal gastrointestinal tract of pregnant albino rats

It is well recognized that radiation can be carcinogenic for a wide variety of tumors, especially, in breast, thyroid, and bone marrow which appear to be radiosensitive. Bone marrow cells [BM] suppress immune cell responses and have beneficial effects in various inflammatory-related immune disorders. The present study is designed to evaluate the possible role of bone marrow to restore certain histopathological and histochemical changes in the fetal gastrointestinal tract of pregnant rats which exposed to gamma rays. The experimental animals were divided into five groups: 1-Control pregnant rats. 2- Group of pregnant rats irradiated with gamma-rays on day 7 of gestation. 3- Group of pregnant rats irradiated with gamma-rays on day 14 of gestation. 4- Group of pregnant rats irradiated with gamma-rays on day 7 of gestation and treated with BM one hour post-exposure. 5-group of pregnant rats irradiated with gamma-rays on day 14 of gestation and treated with BM one hour post-exposure. All the previous groups were sacrificed on day 20 of gestation [1 day prior delivery]. Exposure of pregnant rats to gamma-rays on day 7 or day 14 of gestation showed many pathological and histochemical changes in the fetal gastrointestinal tract. These changes were more pronounced on day 14 of gestation. The changes include increased proliferation in the mucosal layer, increased signs of lymphocytic infiltration and pyknotic nuclei in addition to highly distorted circular muscle fibers. Also, altered collagen, total protein, polysaccharides contents were noted post-irradiation. Bone marrow transplantation post-irradiation improved the tissue architecture which restored it's normal histological and histochemical pictures. It can be concluded that bone marrow transplantation post-irradiation showed somewhat a considerable ability to overcome radiation injuries or damages from the histological and histochemical point of view on day 7 or 14 of gestation in the studied previous fetal tissues although the healing was incomplete on day 7 or 14 of gestation when compared with the control groups

Histoiogical and uitrastructural studies on the effect of Cleome droserifolia plant from Sinai on the liver and kidney tissues of rats

Sinai regions are characterized by the presence of large number of medicinal plants that are highly used in folk treatments but only a small number of these plants have received scientific and medical evaluation to assess their efficacy. Among these plants, Cleome droserifolia plant, which is commonly used in the Egyptian folk medicine for treatment of many diseases. The present study was planned to examine the safety of using an extract of Cleome droserifolia plant. This study focuses on the histopathological and uitrastructural changes induced by the plant extract in liver and kidney of the experimental rats. Cleome droserifolia treatment produced cellular swelling, cytoplasmic granulation with necrotic cells in addition to appearance of patches of depleted glycogen within the hepatic cytoplasm. The glomeruli were hypertrophied with thickening of Bowman's capsule and the renal tubules exhibited damaged epithelial lining cells in addition to the appearance of numerous lysosomes. Such changes in the present study may be due to the toxic effect or accumulation of one or more of the active pharmacological compounds of the plant in the liver or kidney tissues of the treated rats. It is recommended that future studies and chemical analytical techniques are required to separate and purify the main components from the plant responsible for safety and quality assurances for good practices of the plant