Dual effect of cytokinene on root-nodule bacteria and their host legumes, in relation to their symbiosis

The stimulatory effect of cytokinene was also reflected on the growth parameters of the legume plants, measured by the length of roots and stems and dry weight of shoots. This effect was most intensive at the combined treatment of with specific rhizobia. The bioassay test using barley as test plant, also revealed that cytokinene extracted from liquid culture media, of different species of rhizobia, had a positive effect on the retension of chlorophyll by the first leaves of test plant. Rhizobium, leguminosarum was the most effective strain in this respect

Relationship between curled root hairs and nodulation on legume plants in relation to symbiotic N2 fixation and amino acids synthesis

Results showed a positive significant correlation between the curled root-hairs and the number as well as their weight [mg/plant], after 20 and 75 days of sowing, respectively. Nitrogen fertilization [0.3 Ng/pot] in combination with superphosphate [0.50 g/pot] showed the highest increase in percentage of nodules. Glutamic and aspartic were the predominant amino-acids assimilated by the bacterioids in nodules and in the roots of legume plants under study. However, Faba bean showed the highest quantities of these amino acids as well as higher nitrogen content