ABSTRACT
Fruit of Prunus domestica was extracted in ethanol. The ethanol extract was further extracted with two solvents ethyl acetate and chloroform. The crude ethanol extract and two fractions [ethyl acetate and chloroform] were screened for their antibacterial activity using the agar well diffusion method. They were tested against nine bacteria; five Gram positive bacteria [Staphylococcus aureus, Streptococcuc intermedius, Bacillus cereus, Bacillus pumilus] and four Gram negative bacteria [Eschrichia coli, Proteus mirabilis Shigella flexneri, Salmonella typhi and Klebsiela pneumoniae]. The susceptibility of microorganisms to all three fractions was compared with each other and with standard antibiotic [Ampicillin] Among all fractions ethyl acetate exhibited highest antibacterial activity [average zone of inhibition 34.57mm +/- 1.3] while ethyl alcohol exhibited least antibacterial activity [average zone of inhibition 17.42mm +/- 3.3]. Minimum inhibitory concentration of ethanol, ethyl acetate and chloroform fractions was found in the range of 78ug/ml to 2500ugl/ml against gram positive and gram negative bacteria