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ObjectiveTo summarize the method and experience of the model of rat heterotopic heart transplantation and to improve the rate of success of the experiment.Methods120 heterotopic heart transplantations were performed by Ono's way,including 60 prepared transplantations and 60 formal transplantations.ResultsRate of success of prepared transplantations was 45％,and formal transplantation was 85％.Mean time of formal operation was (74.3±17.1)min,removal time of the donor heart was (7.9±4.2)min,ischemia time was (29.6±10.3) min and the survival time of allograft was (11.1±5.7)days.5 of the recipients had a long-term survival,and the maximum weight was 740 g.ConclusionsGood surgical technique and rigorous perioperative management is very important in setting up the rat model of heterotopic heart transplantation.
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Objective To study the effects of administration of donor apoptotic bone marrow cells via portal vein on the survival time of heart transplantation in rats.Methods Wistar rats were selected as donors and SD rats as recipients, and divided into 4 groups at random. In groups A, B and C, the animals were injected via portal vein with RPMI 1640 0.5 ml 6 as control group, 5?107 donor bone marrow cells and 5?107 apoptotic donor bone marrow cells 6 days before transplantation, respectively , and not subjected to cyclosporin A (CsA). In group D, the animals were injected intra- peritoneally with CsA 5 mg/kg from 3 days before transplantation till 10 days post operation. Bone marrow cells apoptosis was induced by 60 Co? radiation. Heart transplantation models were established in each group. The survival time of donor heart and histological changes were observed. Serum concentrations of IL-10 and TGF-?_1 of recipients and mixed lymphocyte reaction (MLR) were measured. Results The survival time of heart allografts in the group C ( 14.00 ? 0.95 ) was significantly longer than in group A (P
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Objective To study the prevention of cardiac allograft vasculopathy (CAV) post-transplant through the expression of iNOS mRNA. Methods Rat model of heterotopic heart transplantation (Abdomen) was developed and recipients were injected with Cyclosporin A at abdomen in the meantime of receiving L-arginine (iNOS mRNA group) and inhibitor of iNOS (L-NAME group) according to grouping. Plasma content of NO_3-, the expression of iNOS mRNA in coronary ~artery of transplanted heart and the radio of tunica intima/tunica media thickness (I/M) were assayed after ~operation . Results The plasma content of NO_3-and the expression of iNOS mRNA in coronary ~artery of transplanted heart in iNOS mRNA group were obviously higher than those in control group and L-NAME group during 2 to 4 weeks after operation. The ratio of I/M in iNOS mRNA group was ~lower than that in control group and L-NAME group 4 weeks after operation. Conclusion The ~expression of iNOS can promote the synthesis of NO and prevent CAV development.