ABSTRACT
Objective To carry out a taxonomic identification of a strain of claviform bacteria iso-lated from prostatic fluid of a patient who suffered from chronic prostatitis, and to approach its phylogenic and biologic position. Methods We undertaked an initial identification by phenotypic characters such as morphologecal, physiological and biochemical characteristics to ascertain its phylogeny by chemical composi-tion analysis of cell wall and 16S rRNA gene sequencing and alignment. Results A club-shaped gram posi-tive rod bacillus was isolated in pure culture state. Its biochemical reactions were not active. The diamino-acid of cell wall was meso-diaminopimelic acid (meso-DAP) and it had wall chemotype Ⅳ ( contained arabi-nose, galactose and maltose ). Sequence searches of the GenBank database revealed that this strain had a highest level of 16S rDNA sequence similarity (99.4%) to C. tuberculostearicurn strain ATCC35692 with only 8 nucleotides difference. Conclusion On the basis of phenotypic and phylngenetie analysis, it is rea-sonable to assign this strain to the species C. tuberculostearicum, and this is the first isolation of C. tubercu-lostearicum from prostatic fluid home and abroad.
ABSTRACT
Objective To investigate a kind of inflammatory pathogen of infectious skin lesion and its position of the bacteria classification. Methods Based on the phenotypic properties of morphology,physiology and biochemical et al.The identification of bacteria was made almost thoroughly and systemically.Results A unique kind of gram-positive bacteria were separated from the lesion of the disease.Which featured by a bacilli to cocci circulation,obligate acrobes,inactive of biochemical reaction and resistance to conventional antibiotics.Conclusion The bacteria are classified into a rare kind of opportunistic pathogen.Few references both at home and abroad has seen so far according to its phenotypic properties,the bacteria is Brevibacterium epidermids which had never been reported at domestic.
ABSTRACT
Nine strains of Serratia rnarcescens were isolated from clinical samples. In the identification, 20 items of biochemical reactions and 124 items of substrate utilization tests have been used. Two strains, which have not been appointed to a biotype of the species, can utilize 3-hydroxybenzoate, 4-hydroxybenzoate or protoeatechuate as sole carbon source for growth. The growth in protocatechuate medium was more rapid as compared with 3 or 4-hydroxybenzoate medium. So, we here suggest that protocatechuate should be included in the substrate utilization for the genus. The other seven strains have been identified as Grimont' s A_2 subphenon of S. marcescens. Pencillin and other wall-acting antibiotics are all resisted by these strains; aminoglycoside antibiotics are almost all effective. Co-trimoxazole and sulfadiazine, though couldn't inhibite the growth of the strains, do inhibite their pigment production.