Detection of hepatitis E virus genome with a thymine-thymine dimerized cNDA probe

The study described the spot hybridization technique using a non-radiolabelled, thymine-thymine dimerized synthetic oligonucleotide complementary DNA(cDNA) proble for the detection of hepatitis E virus genome in bile. The test performed well with high degree of sensitivity and specificity. It was compared with the conventional electron microscopy and was found to be thousand times more sensitive.