ABSTRACT
Phosphopeptide enrichment is of great significance in phosphoproteomics related research. Pre-analysis enrichment helps concentrating phosphopeptides of low abundance, and other interferences of high abundance, such as non-phosphorylated peptides, will be eliminated at the same time. By this way, the enhanced sensitivity of mass-spectrometry analysis of phosphopeptides can be achieved, as well as better detection and identification performance. The key for phosphopeptide enrichment is enrichment materials with specific affinity towards phosphopeptides. Enrichment materials with different phosphopeptide adsorption mechanisms have been developed, while various improvements on material morphologies, enrichment operation, and enrichment specificity are also reported. In the review, we summarized recent progresses in phosphopeptide enrichment from perspectives of both enrichment materials with different enrichment mechanisms, and improvement on methodologies of phosphopeptide enrichment based on various enrichment materials.