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Aim To investigate the expression of IncRNA AC079466. 1 in non-small cell lung cancer (NSCLC) tissues and cells, and the effect of its overexpression on the proliferation, apoptosis, migration and invasion of A549 and H1299 cells. Methods Cancer tissues and corresponding adjacent tissues from 20 NSCLC patients were collected, and the expression of IncRNA AC079466. 1 in tissue and cells was detected by qRT-PCR. AC079466. 1 group was transfected with overexpression plasmid, NC group was transfected with empty plasmid, and no transfection was used in the Blank group. MTT, flow cytometry and Transwell were used to detect the effects of IncRNA AC079466. 1 overexpression on the viability, apoptosis, migration and invasion of A549 and HI299 cells. Western blot was used to detect the effect of overexpression of IncRNA AC079466. 1 on the expression of endoplasmic reticulum stress-related factors GRP78, PERK, eIF2a, ATF4, CHOP, Bax and caspase-3. Results Compared with adjacent tissues, the expression of IncRNA AC079466. 1 in cancer tissues significantly decreased. Compared with HBE cells, the expression of IncRNA AC079466. 1 significantly decreased in A549 and H1299 cells. Compared with the Blank group and NC group, the viability, migration and invasion abilities of A549 and H1299 cells in AC079466. 1 group all markedly decreased, the apoptosis rate apparently increased, and the expressions of endoplasmic reticulum stress-related factors GRP78, p-PERK, eIF2a, ATF4, CHOP, Bax and caspase-3 were significantly up-regulated. Conclusion The overexpression of IncRNA AC079466. 1 significantly inhibits the viability, migration and invasion of A549 and HI299 cells, and promotes cell apoptosis. The mechanism may be related to the promotion of endoplasmic reticulum stress-mediated cell apoptosis.
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@#Objective To investigate the relationship between gait spatiotemporal parameters and balance function in stroke hemiplegic patients. Methods From September, 2018 to March, 2020, 76 stroke hemiplegic patients (38 in Brunnstrom Ⅳ and V, respectively) were assessed balance function with Active Balancer EAB-100 and Star Excursion Balance Test (SEBT), and gait parameters of speed, cadence and asymmetry, named step length asymmetry ratio (SLA), swing time asymmetry ratio (SWTA), stance time asymmetry ratio (STA), maximum flexion angle ratio (MFA) of hip and knee. The correlation among gait parameters and balance function was analysed. Results The balance and gait parameters were better in those in Brunnstrom V than in Brunnstrom Ⅳ (|t| > 2.268, P < 0.05). The speed and cadence correlated with the most balance indexes (|r| > 0.335, P < 0.05). SLA correlated with SEBT in Brunnstrom Ⅳ patients (r = -0.467~-0.613, P < 0.01), while it correlated with medial-lateral (M-L) stability limits in Brunnstrom V patients (r = -0.356, P = 0.028). SWTA correlated with stability limit of Anterior-Posterior (A-P) (r = -0.335, P = 0.040) and M-L (r = 0.510, P = 0.001), and most of SEBT (r = -0.330~ -0.445, P < 0.05) in Brunnstrom Ⅳ patients. MFA of hip and knee correlated with balance indexes in Brunnstrom Ⅳ patients (|r| > 0.326, P < 0.05), while MFA of knee correlated with most balance indexes in Brunnstrom V patients (r > 0.329, P < 0.05), and MFA of hip correlated with A-P stability limit (r = 0.369, P = 0.023). Conclusion Both speed and cadence of steps can well respond to the balance function in stroke hemiplegic patients. For the asymmetry, it is related with balance in patients with poorer recovery.
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In recent years, micro/nano-scaled separation technique has attracted increasing attention due to its inherent advantages. The porous layer open tubular ( PLOT) capillary column is an important microcolumn form. Comparied to wide-bore PLOT capillary columns ( inner diameter >25 μm ) , the PLOT capillary columns with a narrow inner diameter yield higher separation efficiency and lower reagent consumption. In this paper, the preparation methods for porous layer open tubular capillary columns with narrow inner diameter, less than or equal to 25 μm, are reviewed. Detection techniques combined with mass spectrometry and their applications in liquid chromatography are also disussed.
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Objective To evaluate the effects of unsupported arm exercise in patients with chronic obstructive pulmonary disease.Methods Databases such as Cochrane Library,PubMed,EMbase,CNKI and WanFang were searched to recruit eligible randomized controlled trials.The effects of unsupported arm exercise was determined by meta-analysis with RevMan 5.3 after data extraction and quality appraisal.Results Totally 11 RCTs were finally recruited.The results of meta-analysis supported the effectiveness of unsupported arm exercise on improving dyspnea in patients with chronic obstructive pulmonary disease[WMD=-0.76,95％CI(-l.33,-0.20),P=0.008],as well as the effectiveness on enhancing upper arm activity [WMD=25.75,95％CI (10.45,41.05),P=0.0010] and exercise tolerance [WMD=33.98,95％CI (10.20,57.76),P=-0.005].While it failed to support the effectiveness on improving lung function and quality of life(P＞0.05).Conclusion Unsupported arm exercise is effective to improve dyspnea,enhance upper arm activity and exercise tolerance,and is worth introducing in rehabilitation exercise in patients with chronic obstructive pulmonary disease.However,the effects on lung function,quality of life are still inconclusive.Strict designed,multi-centered RCTs with large sample size are needed in the future to gain reliable effectiveness of unsupported arm exercise.
ABSTRACT
Objective To evaluate the effects of unsupported arm exercise in patients with chronic obstructive pulmonary disease.Methods Databases such as Cochrane Library,PubMed,EMbase,CNKI and WanFang were searched to recruit eligible randomized controlled trials.The effects of unsupported arm exercise was determined by meta-analysis with RevMan 5.3 after data extraction and quality appraisal.Results Totally 11 RCTs were finally recruited.The results of meta-analysis supported the effectiveness of unsupported arm exercise on improving dyspnea in patients with chronic obstructive pulmonary disease[WMD=-0.76,95％CI(-l.33,-0.20),P=0.008],as well as the effectiveness on enhancing upper arm activity [WMD=25.75,95％CI (10.45,41.05),P=0.0010] and exercise tolerance [WMD=33.98,95％CI (10.20,57.76),P=-0.005].While it failed to support the effectiveness on improving lung function and quality of life(P＞0.05).Conclusion Unsupported arm exercise is effective to improve dyspnea,enhance upper arm activity and exercise tolerance,and is worth introducing in rehabilitation exercise in patients with chronic obstructive pulmonary disease.However,the effects on lung function,quality of life are still inconclusive.Strict designed,multi-centered RCTs with large sample size are needed in the future to gain reliable effectiveness of unsupported arm exercise.