ABSTRACT
Here in this paper we have reported the extracellular biosynthesis of silver nanoparticles by using filamentous fungi Penicillium sp. The Penicillium sp. was isolated from the soil sample collected from vegetable market in Chennai, Tamil Nadu, India. The purified fungal culture was subjected for the biosynthesis of silver nanoparticles. The color change of the solution in the conical flask into dark brown suggests the formation of silver nanoparticles(AgNPs). These Silver nanoparticles have been further characterized by UV-vis spectrophotometer which showed the absorption peak at 416nm which confirms the nanoparticles synthesis, Fourier Transform Infrared (FTIR) Spectroscopy showed proteins and functional groups which stabilizes the nanoparticles as capping agent , Xray diffraction (XRD) analysis determine the crystalline nature of silver nanoparticles and Field emission scanning electron microscopy (FESEM) analysis which showed the particle size was around 35 to 67 nm and. These biologically synthesized silver nanoparticles showed good antibacterial activity against the selected bacterial pathogens and also these nanoparticles enhanced the antibacterial property of Sparfloxacin and Ofloxacin.