Evaluation of nucleolar organizer region in normal brain tissues and astrocytic gliomas

This study was performed to evaluate the importance of Ag NORs in different grades of Astrocytic Gliomas. It was a descriptive study and was performed at Lahore General Hospital and Sheikh Zayad Hospital, Lahore. A total of 50 cases were collected within one year, and AgNOR staining was performed on 50 brain tumors and specimens were selected randomly. AgNOR was low, high, higher and highest in Astrocytic Gliomas, grade I [Pilocytic], II, III, and IV respectively as compared to normal brain tissue. AgNOR staining technique is a simple, quick and a cheap method to assess proliferative index in astrocytic Proliferative activity is a good parameter to assess the disease outcome in astrocytic glioma

Role of anti microbial agents in delaying changes of autolysis

Background: Formalin is the most widely used tissue fixative. However, it renders tissue unfit for immunohistochemistry by masking most of the commonly useful antigens. Other organ preservation solutions should be tried. To assess the ability of tissue preservation in Ringer lactate, Euro Collins solution [EC solution] and University of Wisconsin solution [UW solution], with and without antimicrobial agents. Experimental study on rabbit renal tissue. Main. outcome measures: Histological examination of the sections to assess preservation by a semi quantitative method. UW solution was found to be the best preservation solution.. EC solution was second, followed by Ringer lactate. The addition of anti microbial agents improved the preservation in the latter two solutions markedly. Ringer lactate with addition of anti microbial agents was found to be a cost effective alternative. Immunohistochemistry should be tried on tissues submitted in Ringer lactate with anti microbial agents