Histopathological and histochemical studies of the ovary and uterus of the female dog after ligation of the supplying arteries

The present study was undertaken to study the histopathological and histochemical studies of the ovary and uterus of female dog after ligation of the supplying arteries. Twelve adult female dogs were divided into three groups. Unilateral ligation of the internal iliac, uterine and ovarian arteries was done in group I, II and III, respectively. All animals were sacrificed one month after the operation. Samples were taken from the ovaries and uterine horns of both the ligated and nonligated sides for histological and histochemical studies. Unilateral ligationof either internal iliac, uterine or ovarian arteries was followed by histopathological changes in the uterine horns of both sides. However, the degenerative changes were more marked at the horns of the ligated sides. The endometrial glands of both uterine horns showed negative PAS and alkaline phosphatase activies after unilateral ligation of the internal iliac or uterine arteries. However, after ligation of the ovarian artery, the endometrial glands of the ligated side showed weak PAS reaction and moderate alkaline phosphatase activity. Those of the contralateral side showed a strong activity. Concerning the ovary, unilateral ligationof the internal iliac or uterine arteries was followed by marked degenerative changes in the ipsilateral ovary and the contralateral ovary was normal in appearance. However, ligationof the ovarian artery was followed by atrophic changes in the ovary of the ligated side. That of the contralateral side showed compensatory hypertrophy of the graafian follicles. Ligation of the internal iliac or uterine arteries was followed by strong activity of the alkaline phosphatase enzyme in the growing follicles and corpus lutii of both ipsilateral and contralateral ovaries. There was also a strong activity of the alkaline phosphatase in the hypertrophied follicles of the contralateral ovary following ligation of the ovarian artery

Effects of ambilhar on the pregnant female rats and their neonates

The present work was undertaken to study the effect of ambilhar [antibilharzial drug] on the pregnant female rats and their necnates. Ten pregnant female rats were used in this work. They were divided into five experimental and five control animals. From the eighth till the fourteenth day of gestation, the experimental animals were given a daily oral dose of 6 mg Niridazole [ambilhar] dissolved in normal saline. All the rats were allowed to deliiver. The mothers and their neonates of both the experimental and the control groups were killed on the first day of delivery. The heart, liver, kidney and spleen were tajen for the histological and histochemical studied. Administration of ambilhar were followed by marked degenerative changes in the organs under investigation. Thansplacental passage was also proved in the present study as many histopathological changes were observed in the fetal organs. There were also a decrease in the activity of the alkaline phosphatase enzyme and the reaction of PAS. The significance of these findings was discussed