Egyptian genotyping of Giardia lamblia

In this study, out of 105 patients infected with Giardia, 38 patients had genotype I, 13 had genotype II, 10 had genotype III, 16 had mixed genotype infection and 28 with undetermined Giardia infection by polymerase chain reaction [PCR]. None of the control group gave positive results for Giardia in stool by PCR. So, the sensitivity of the test for the detection and identification of Giardia genotypes from the original stool samples was 73.33% and the specificity was 100%. Out of 61 cases in the symptomatic group, the prevalence of Giardia genotype I was 32.79%, genotype II was 16.39%, genotype III was 9.84%, mixed genotype infection was 16.39% and undetermined genotype was 24.59% as compared with 40.91%, 6.82%, 9.09%, 13.64% and 29.55% in the asymptomatic group, respectively. There was a statistically insignificant difference between both groups regarding the prevalence of the different Giardia genotypes

Evaluation of two serological tests in diagnosis of human cases of biliary and ectopic fascioliasis

In the present study, sera were examined using Fasciola-IHA, F-ELISA and Schistosoma-IHA. The seropositive schistosomal patients were subjected to rectal snip, while the seropositive fascioliasis ones were examined radiologically by plain chest X-ray and abdominal ultrasonography. Both F-IHA and F-ELISA gave 100% sensitivity. The specificity of both tests was 90.4% and 100%, respectively. With the S-IHA, the parasitologically proven schistosomal mansoni patients gave 95% positivity; while, the proven fascioliasis patients gave false positivity [8%] with this S-IHA. The radiological findings of the fascioliasis patients confirmed ectopic pleuropulmonary infection in five patients. Ascitis was detected in one patient. The pleural effusion and ascitic fluids showed high eosinophils. However, Fasciola eggs were detected in three patients only. Two patients showed hepatic nodules and another two had hepatic cystic lesions. Three patients had immature Fasciola worms in gall bladder. For the diagnosis of human fascioliasis, serological and radiological means should be done together with the stool examination. This is particularly true in chronic cases and in schistosomiasis mansoni endemic areas

woman and her son with abnormal complicated anthrponotic cutaneous leishmaniasis

This paper presented a son and his mother with positive leishmaniasis IHAT [1/128 and 1/1924, respectively]. Using enzymatic electrophoresis characterization, the two isolates proved to be Leishmania tropica that causes anthroponotic cutaneous leishmaniasis [ACL]. The son had two large ulcerative ACL on his left hand. The mother had a large ulceration and progressive erosion of the soft tissue [the right cheek and right eye] and the cartilage of the nose disfigured and debilitated her face. The histopathological examination of the biopsy material obtained from the mother, but not the material from her son, showed typical pathological picture of the basal cell carcinoma