Efficiency of combining pomegranate juice with low-doses of cisplatin and taxotere on A549 human lung adenocarcinoma cells

Objective: To test the coalescence effect of two chemotherapy drugs at low effective dose (cisplatin and taxotere) combined with pomegranate juice on A549 cancer cells. Methods: Infrared spectroscopy method is a qualitative test that was performed to ensure the existence of the phytochemicals providing the antioxidant activity through the presence of the hydroxyl group (-OH). The viability of A549 cell line and normal MCs was tested using the neutral red uptake, Clonogenic survival, XTT and Cell migration assays. Results: Our results showed that this combination firstly led to a greater decrease in the viability of cells comparing to those treated with chemotherapy drugs alone, and secondly led to a significant reduction in cell migration. Conclusions: These data suggest a synergistic effect between the pomegranate and cisplatin which makes probably this combination a powerful option for treating lung adenocarcinoma and in parallel minimizing the systemic side effects.

Embodiment of religious figures in the Iranian drama and relationship to mental image in adolescents

Problem: This study discuss the following question: What is the relationship between the embodiment of religious figures in the Iranian drama and the mental image of them in adolescents?

Objectives:Identify the habits and patterns and density View teenagers Iranian drama which embody religious figures and motives of watching her, Knowledge of the mental image in adolescents for religious figures, and Come to know the relationship between the embodiment of religious figures in the Iranian drama and the mental image of them in adolescents

Type and Methodology: The study is belonged to descriptive studies, survey method was used

Sample: A sample of adolescents to examine the relationship between watching the Iranian drama which embodied religious figures and formed a mental picture they have from those figures seen through, and Field sample applied on a stratified random sample of 400 single-strong adolescents [Male/ Female] in Cairo Governorate [High School] who are watching the Iranian drama that embodied the religious figures

Instruments: Questionnaire Form: to collect data under the frame of survey methodology by sample

Results: That 83.4% of respondents reject the embodiment and the emergence of religious figures [Prophets-Companions] in the Iranian drama, while 9.9% were neutral, and in contrast, 6.7% of them agree to the embodiment and the emergence of religious figures [Prophets-Companions] in the Iranian drama. That 63.4% of me respondents do not accept the image that embodied the religious figures in Iran's drama, while 31.6% of them accept them sometimes, and 5.1% of them accept them always. 43% of the respondents, first thing remember him when he comes respondents said religious figure, which looked at is a personal drama that appeared in this business sometimes form, while in contrast, 26.7% of them remember the other things, and 30.2% of them remembers always. Test the validity of hypotheses, the first hypothesis is proven wrong: no statistically significant correlation between the reflected image for the embodiment of religious figures and intensity of exposure. There were statistically significant differences between the respondents about the respondents believe that religious figures provided in the Iranian drama figures are close to the real figures depending on the type

Expression of e-cadherin, n-cadherin and snail and their correlation with clinicopathological variants: an immunohistochemical study of 132 invasive ductal breast carcinomas in Egypt

OBJECTIVE: To evaluate the expression of the cell adhesion molecules E-cadherin and N-cadherin and the transcription factor Snail in invasive ductal breast carcinomas and to determine their relationships with clinicopathological features. METHODS: Immunohistochemistry was used to examine E-cadherin, N-cadherin, and Snail protein expression in 132 invasive breast carcinomas. RESULTS: The expression of E-cadherin was decreased (negative or weak) in 37.1 percent of invasive carcinomas, while N-cadherin and Snail overexpression were detected in 51.9 percent and 40.9 percent of carcinomas, respectively. Low E-cadherin expression was significantly correlated with poorly differentiated carcinoma (53.1 percent), positive node status (80.9 percent), poor Nottingham Prognostic Index (64.7 percent), and the presence of estrogen and progesterone receptors. Overexpression of N-cadherin and Snail were also significantly correlated with poorly differentiated carcinoma, positive node status, and poor Nottingham Prognostic Index but were correlated with the absence of hormone receptors. Loss of E-cadherin immunoexpression was strongly associated with the presence of membranous N-cadherin (87.8 percent) and nuclear Snail (69.4 percent). CONCLUSION: Loss of E-cadherin and overexpression of N-cadherin and Snail in breast carcinomas may play a central role in the development of invasive ductal breast carcinoma. These biomarkers may provide a valuable reference for the study of invasive ductal carcinoma progression and to characterize the biological behavior of the tumor. In the future, increased N-cadherin and decreased E-cadherin expression may be used as indicators of the progression and prognosis of invasive ductal carcinoma.

Immunocytochemical localisation of heme oxygenase isoforms in normal and cirrhotic human liver

Heme oxygenase [HO] is the rate-limiting enzyme in the catabolism of heme to biliverdin, iron and carbon monoxide. Two isoforms of HO have been characterized: the constitutive isoform [HO-2], the major isoform present under physiological conditions, and the stress-induced isoform [HO-1], which is also known as heat shock protein. In this study 15 liver biopsies from patients with cirrhosis secondary to hepatitis B and C and 5 biopsies from healthy controls were investigated for the expression of HO-1 and HO-2 protein using a standard immunohistochemical method. In normal liver, HO-1 immunoreactivity was seen in Kupffer cells. In cirrhotic liver, increased HO-1 immunoreactivity was seen in Kupffer cells, the endothelial lining of portal vein and hepatic sinusoids, and to a lesser extent, in hepatocytes. In Kupffer cells, dense perinuclear HO-1 reactivity was seen, whereas in hepatocytes HO-1 reactivity was weak and diffuse throughout the cytoplasm. By comparison with control tissues, only HO- 1 showed significant increase immunoreactivity in cirrhotic liver [p<0.04] On other hand, HO-2 immunoreactivity was seen only in hepatocytes in both control and cirrhotic livers. This result shows that in liver cirrhosis, HO-1 expression is increased significantly and is seen preferentially in Kupffer cells. This suggests that Kupffer cells may act as sensory cells that detect the elevation of intrasinusoidal pressure accompanying hyperdynamic states secondary to portal hypertension, implicating an increase in regional wall stress in sinusoids and thereby alter the ability of Kupffer cells to degrade heme through HO-1 induction. Another mechanism by which HO- 1 could be upregulated in liver cirrhosis is via nitric oxide, and we have previously shown high expression of NO in liver cirrhosis. This study suggests a role for HO- 1 in the development of ci1Thosis in viral hepatitis and points out the need for additional work in this area