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1.
Arq. bras. med. vet. zootec ; 61(5): 1173-1182, out. 2009. tab, ilus
Article in English | LILACS | ID: lil-532030

ABSTRACT

This study was carried out to morphologically characterize and classify the stages of gonad development in different Nile tilapia strains (Oreochromis niloticus). Eighty-four and ninety-two ovaries from Bouaké and Chitralada strains, respectively, were evaluated at different ovarian developmental phases: initial (104 days of culture), intermediate (152 days of culture), and the final (279 days of culture). The ovaries were microscopically evaluated and submitted to histological processing and hematoxylin-eosin staining to determine their characteristics and be classified. No morphological differences in ovaries between strains were observed during the initial phase (stage A - immature). During the intermediate growing phase, higher gonad development was observed for Chitralada strain (stage B - maturation) in comparison with Bouaké strain (stage A - immature). During the final growing phase, no differences between strains were observed for morphological characteristics (stage C - mature). Despite the similarities in reproductive behavior of the Bouaké and Chitralada females at the end of the final growing phase (gain weight phase), differences for macroscopic and microscopic aspects and oocytes during the initial and intermediate growing phases of the strains were observed.


Este trabalho teve como objetivo caracterizar morfologicamente e classificar os estádios de desenvolvimento gonadal de tilápias do Nilo (Oreochromis niloticus) de linhagens distintas. Foram avaliados 84 ovários da linhagem Bouaké e 92 da linhagem Chitralada, em diferentes fases de desenvolvimento: inicial (imatura - 104 dias de cultivo), intermediária (crescimento - 152 dias de cultivo) e final (ganho de peso - 279 dias de cultivo). Os ovários foram analisados macroscopicamente e submetidos a procedimento histológico, corados com hematoxilina-eosina, para determinação das características microscópicas e subsequente classificação. Não foram observadas diferenças morfológicas nos ovários entre as linhagens na fase inicial (estádio A - imaturo). Na fase intermediária, os ovários revelaram diferenças morfológicas, evidenciando maior desenvolvimento gonadal para a linhagem Chitralada, (estádio B - maturação) em relação à Bouaké (estádio A). Na fase final (ganho de peso) os ovários não apresentaram diferenças morfológicas entre as linhagens (estádio C - maduro). Apesar de semelhanças no comportamento reprodutivo das fêmeas das linhagens Bouaké e Chitralada, na fase final (ganho de peso), diferenças, em relação à classificação da fase intermediária nos aspectos macroscópicos e microscópicos e diâmetro dos ovócitos, foram observadas.


Subject(s)
Animals , Oocytes/growth & development , Ovary/anatomy & histology , Cichlids , Ovary/growth & development , Reproductive Behavior
2.
Biocell ; 31(1): 33-39, abr. 2007. ilus, tab, graf
Article in English | LILACS | ID: lil-491535

ABSTRACT

Alterations in the gastrointestinal neuromuscular function related to age have been demonstrated in human and animal models. This study analyzes the effects of the aging process on the area of the neuronal cell bodies of the myenteric plexus in the antimesenteric and intermediate regions of the ileal circumference of Wistar, 12 month-old in comparison 3 month-old animals. The ileum was removed and whole-mount preparations immunostained by the antibody anti-myosin-V were processed. The morphometric analyses were performed using a computerized image analysis system, with a subsequent distribution of neurons by size in intervals of 100 micro2. The cellular body morphometry revealed a significant increase in the size of the myosin-V- immunoreactive myenteric neurons from 12 month-old animals when compared with 3 month –old animals. However, significant differences between the regions were not observed; these observations were not age-dependent. The implications of these results in relation to the increase of the body weight, size of the small intestine, general organization of the myenteric plexus, staining method of neurons and the possible factors involved in the regulation and/or control of the volume of neronal cells due to aging, are discussed.


Subject(s)
Animals , Male , Rats , Aging , Ileum/innervation , Myosin Type V/analysis , Myosin Type V/immunology , Neurons/cytology , Neurons/chemistry , Myenteric Plexus/cytology , Immunohistochemistry , Rats, Wistar
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