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Rev. cient. (Maracaibo) ; 20(3): 227-234, jun. 2010. ilus, tab
Article in Spanish | LILACS | ID: lil-631064

ABSTRACT

El objetivo del presente estudio fue determinar la población bacteriana vaginal de la vaca Criollo Limonero. Se tomaron muestras que consistieron en hisopados por duplicado a nivel del fornix de la vagina anterior, las cuales se sometieron a cultivos bacterianos en ambiente aerobio/anaerobio y pruebas bioquímicas convencionales para el aislamiento. De 102 hisopados se lograron 55 crecimientos (53,9%) y 47 sin crecimiento (46,1%). Los 55 crecimientos se distribuyeron de varias maneras: 23 (41,82%) aerobios y 32 (58,18%) anaerobios; 29 (52,72%) aislamientos puros y 26 (47,27%) aislamientos mixtos. Las bacterias Gram positivas predominaron (81,82 y 73,08%), con respecto a las Gram negativas (18,18 y 26,92%), tanto en aerobiosis como en anaerobiosis, respectivamente. Las bacterias más aisladas fueron Arcanobacterium pyogenes (22,92%), Staphilococcus aureus (15,63%), Staphylococcus coagulasa negativo (17,71%), Erysipelothrix rhusiopathiae (6,25%), Bacteroides spp. (13,54%) y Peptostreptococcus spp. (7,29%). En conclusión, se identificaron las bacterias que forman parte de la flora bacteriana vaginal de la vaca Criollo Limonero, su distribución en cuanto a especie, género, exigencia de oxígeno, tipo de aislamiento, tinción de Gram y potencial de patogenicidad son discutidos.


The objective of the current study was to determine the population of vaginal bacteria in Criollo Limonero cows. Two swabs from every cow were taken at the level of the fornix for bacterial isolation with biochemical test. From 102 swabs, 55 achieved bacterial growth (53.9%) and 47 did not get to any bacterial growth (46.1%). The 55 samples that achieved bacterial growth were distributed as follows: 23 (41.82%) aerobic bacteria and 32 (58.18%) anaerobic bacteria; 29 (52.72%) pure isolations and 26 (47.27%) mixed isolations. Gram positive bacteria were predominant (81.82 and 73.08%) compared to Gram negative bacteria (18.18 y 26.92%), both in aerobic and anaerobic media, respectively. Predominant bacteria were Arcanobacterium pyogenes (22.92%), Staphylococcus aureus (15.63%), Staphylococcus coagulase negative (17.71%), Erysipelothrix rhusiopathiae (6.25%), Bacteroides spp. (13.54%) and Peptostreptococcus spp. (7.29%). In conclusion, vaginal bacterial flora from Criollo Limonero cows were identified, in reference to the distribution of their specie, genus, oxygen requirements, type of isolation, Gram stain and potential pathogenic capacity were discussed.

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