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Noble bimetallic nanoparticles (BNPs) exhibit strong anticancer and antibacterial activities. They are known to be stable, less toxic, environmental friendly and biocompatible nature. Due to their enhanced biological properties, they are well suits for the biomedical applications such as cancer therapy, gene therapy and drug delivery. In the current study, we examine the anticancer and antibacterial potential of bio-mediated mono and bimetallic nanoparticles. Here, aqueous Capsicum annuum leaf extract was employed as a good reducing and capping agent for producing Ag, Au monometallic, and Ag-Au bimetallic nanoparticles. The formation of C.A- Ag, Au MPNs and Ag-Au BNPs was initially confirmed by visible color change of the reaction mixture and UV-Visible spectra show the Surface Plasmon Resonance (SPR) band observed at 543 nm. Furthermore, phytofabrication, crystallinities, structural alignments, particle size and elemental composition were studied by following standard physico-chemical techniques such as FT-IR (Fourier Transform Infrared), XRD (X-Ray diffraction), HR-TEM (High resolution-Transmission Electron Microscopy), SEM-EDX (Scanning Electron Microscopy and Energy- dispersive X-Ray Spectroscopy) respectively. The results were obtained from various characterization techniques confirmed that the C.A. mediated Ag, Au MNPs and Ag-Au BNPs were spherical in shape and FCC structure with nanoscale range (10-25 nm). The BNPs exhibit strong efficacy against bacterial strains. These nanoparticles were subjected to investigate the anticancer activity against human lung cancer cells (A549 cell line) through MTT assay. The cell viability was determined by this assay. The occurrence of cell apoptosis and necrotic were quantified by using dual fluorescent staining (AO/EB) and flow cytometry analysis. However, Ag-Au bimetallic nanoparticles showed highest cytotoxic potential with low IC50 - 57.35 ± 0.05 µM values. This IC50 value is comparatively lower than, Ag, Au MNPs and C.A. aqueous leaf extract. IC50 values of C.A- Ag-Au BNPs predominantly induced the cell apoptosis, necrotic and the death of A549 cells suggested the anticancer potential of C.A. mediated Ag-Au BNPs to treat the lung cancer cells.
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With the application of intensive agricultural techniques, conventional agriculture has been successful in meeting production goals but has also led to the depletion of natural resources. Sustainability in the management of the natural resource base is necessary for ongoing and expanded agricultural output to provide food security for future generations. Over a long period, the traditional tillage practice has led to the destruction of the natural resource base of the land. As a result of its inherent connection to the physical, chemical, and biological aspects of soil, it has led to a significant loss of soil and SOM, which is a crucial component of soil quality. Therefore, a suitable cropping system and land use must be implemented and adopted to ensure food security on a sustainable basis. These measures should be based on principles to prevent land degradation, protect the natural resource base, and improve food and nutritional security through crop diversification and optimal rotation. The one sustainable cropping method that may reverse soil erosion, increase crop output, and improve the socioeconomic status of small landholder farmers is conservation agriculture (CA). Conservation agriculture (CA) is the integrated management of the available natural resources such as soil, water, flora, and fauna, with certain outside inputs to maximize the effectiveness of natural resource utilization. To fulfill the objective of sustainable crop production, an alternative approach known as conservation agriculture has emerged. It represents a significant advance in the direction of sustainable agriculture. This article reviews the emerging concerns due to the continuous adoption of conventional agriculture systems, contrasting features between conventional and conservation agriculture systems, and various principles and practices in conservation agriculture. It also highlights the benefits and limitations of CA and various challenges in the adoption of CA.
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Bimetallic nanoparticles (BNPs) have gained significant attention in the field of biomedical and pharmaceutical because of its tunable size, shape, high surface to volume and enhanced biological properties. In this study, we report the ecofriendly route to produce the noble (Ag-Au) bimetallic nanoparticles using aqueous flower extract of Clitoria ternatea as a reducing and capping agent. The synthesized C.T-Ag-Au BNPs were characterized by using physicochemical techniques such as UV-visible, FTIR, XRD and SEM-EDX analysis. The UV-visible spectra reveal the formation of homogeneous bimetallic nanoparticles by the single blue-shifted peak at 541 nm of C.T-Ag-Au BNPs. The phyto fabrication of synthesized C.T-Ag-Au BNPs was analyzed using FTIR spectroscopy. XRD confirms the formation of phase pure cubic Ag-Au alloy bimetallic nanoparticles with crystallite size is 14.5 nm. The surface morphology and elemental analysis of C.T-Ag-Au BNPs were examined by using SEM-EDX analysis. The synthesized C.TAg-Au BNPs were evaluated for their cytotoxicity against A549 human lung cancer cells through standard MTT assay. From this assay, green synthesized bimetallic nanoparticles induced cell apoptosis, suggesting that the synthesized C.T-Ag-Au BNPs gave best anticancer properties against lung cancer A549 cell lines.
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Purpose: To establish the normative data of the head and face measurements needed to design an appropriate spectacle frame for the Indian population. Methods: Indian subjects between 20 and 40 years were included in the study. Thirteen parameters were measured using the direct and indirect methods using ImageJ software. Photographs of subjects were captured in the primary gaze position, with the head turned toward the subject’s right and left by 90° from the primary gaze. Results: The mean ± standard deviation of age was 27.6 ± 5.7; 55.38% were males. An independent t?test showed a significant difference in nose width (P = 0.001), inter?pupillary distance (P = 0.032), and body mass index (P = .012) between males and females. Inner inter?canthi distance (P = .265), outer inter?canthi distance (P = .509), and frontal angles (P = .536) showed no significant difference. There is a significant difference in face width compared with the other studies. The mean head width of males (154.168 ± 9.121) was wider than that of females (145.431 ± 8.923). This suggests a smaller distance between the temples of a spectacle frame for females. Conclusion: Considering the above factors, there is a need for a customized spectacle frame design providing better optics, improved cosmesis, and comfort to the wearer
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Background: Urinary tract infection is a common infection among both genders there was higher prevalence among women probably due to the physiology of urinary tract in women. In addition, age is an important factor where elderly people with urinary devices like catheters are prone to the infection. Aims and Objectives: Study conducted to assess the clinical profile, predisposing factors, uropathogen profile causing Urinary tract infection in elderly women and to identify associated factors responsible for it. Methods: The study was conducted in, S Nijalingappa Medical College and HSK Hospital, Bagalkot, Karnataka and was conducted in the Department of Internal Medicine. Study period extended from December 2018 to May 2020. It was a study on elderly women patients (age>60 yr) with symptoms of UTI admitted in HSK hospital. Patients were selected based on inclusion and exclusion criteria. Total number of 65 patients were taken for the study. Results: In our study, UTI commonly witnessed in the age group was 60-69 years. Most common causative organism is E. Coli isolated in 47.7% cases. The average length of hospitalization was 8.65 ± 8.94 days. Most organisms are sensitive to Nitrofurantoin (43%). Conclusion: 1. Urinary tract infection is common infection among both genders there was higher prevalence among women, age is an important factor where elderly people with urinary devices like catheters are prone to the infection. 2. Diabetes enhances the incidence due to elevated blood sugar levels and has a significant role in the incidence of the infection. 3. As gram negative isolates shows drug resistance, prompt diagnosis and the right choice of antimicrobials can play a key role in reducing mortality in elderly UTI patients.
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Financial statements are the pulse of an organization to understand the healthy financial position of an organisation. This Study was taken up in Tamilnadu Industrial Development Cooperative Bank (TAICO), Chennai with the objective to determine the variation in the items of income statement and balance sheet by common size statement, to evaluate the performance of the bank by comparative study and to ascertain the solvency, liquidity and profitability ratios of the bank. For the purpose of the study, last three years financial statements were obtained from the bank.With the help of Common size balance sheet, Comparative Balance sheet and Ratios of Profitability, Solvency and Liquidity the financial status was obtained. The bank has seen a down side during the pandemic period as visible from the analysis. It is also very positive to note that deposits has risen and the bank is close to pre-pandemic status as per 2021 reports. The bank has to concentrate on investing in Fixed assets and also ensure that profits are used in this regard. Other variables indicate a healthy financial statement of the bank
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Objectives@#Children with attention-deficit/hyperactivity disorder (ADHD) tend to be noisy and violate rules with their disruptive behaviors, resulting in greater difficulties with off-task behaviors and being at risk for social refusal. The visual activity schedule (VAS) intervention program is a frequently used method to teach multiple skills involving on-task, use of schedules, transition behaviors, social initiation, independent play skills, classroom skills, and academic skills. The current systematic review aimed to examine the efficacy of using VAS intervention in reducing problem behaviors in children with ADHD between 5 and 12 years of age. @*Methods@#Systematic searches were conducted using two electronic databases (PubMed and Scopus) to identify relevant studies published in English between 2010 and 2020. Four studies met the inclusion criteria: two studies examined the effect of schedule-based tasks and the use of an iPad on classroom skills, while the other two examined randomized clinical trials (RCTs) of psychosocial treatment for ADHD inattentive type and a cross-sectional study examined the impact of the group size on task behavior and work productivity in children with ADHD. @*Results@#The findings indicate that the interventions used in all four studies could lead to increased satisfaction among participants and parents, as well as a reduction in problem behavior. In terms of the research indicators, the RCT had low quality, while the others were of high quality. @*Conclusion@#A larger number of studies and the ADHD clinical population would help to increase the generalizability of future reviews of treatments in this context.
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Introduction: Spleen is a vital lymphatic organ located in the left hypochondrium of the human body. Morphological variationsof spleen and its clinical importance need to be discussed at dissection tables during routine anatomy dissection hours.Purpose: The aim of this study is to assess the anatomical variations of spleen by its morphometric analysis in adult humancadavers with a possible discussion on its clinical significance.Materials and Methods: A total of 20 adult human cadaveric spleens were studied at ESIC Medical College, Sanathnagar,Hyderabad, Telangana State, India, in a duration of 3 consecutive academic years, 2016–2019. The present study was basedon observing morphological features such as shape, number of notches, and analyzing morphometric measurements such aslength, width, and thickness of all spleens.Results: Shapes of all the spleens were observed and it was found that 40% of spleens were triangular shaped with the highestfrequency. Splenic notches were found majorly on superior border, with their number ranging between 1 and 4. Morphometricvalues of splenic lengths varied from 8.5 cm to 21cm, widths 4 cm to 8.5 cm, and thickness varied from 2.5 cm to 6.5cm.Conclusion: The present study brings a clear concept on morphological and morphometric variations of spleen, which possiblyenhances the basic as well as clinical knowledge of medical professionals.
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Background & objectives: Inflammatory processes are a recognized feature of atherosclerotic lesions. Ranolazine inhibits the inflammatory markers such as C-reactive protein, interleukins-1 and -6 and tumour necrosis factor-alpha. The present study was planned to evaluate the effect of anti-inflammatory activity of ranolazine in acute and sub-acute models of inflammation in rats and compare the same with that of control (gum acacia 1%) and aspirin (standard anti-inflammatory drug). Methods: Adult male Wistar rats (150-180 g) were used for the study. They were divided into three groups (n=6). One per cent gum acacia (control), aspirin (200 mg/kg body weight) and ranolazine (180 mg/kg body weight) were given orally. Acute inflammation was induced by injecting carrageenan in the left hind paw. Paw oedema volume and percentage inhibition were measured. Subacute inflammation was induced by implanting foreign bodies subcutaneously. Percentage inhibition of granuloma dry weight and haematoxylin and eosin stained sections of granulation tissue were studied. Results: In acute and subacute model study, ranolazine significantly (P <0.01) decreased the paw oedema volume and granuloma dry weight as compared to control and it was comparable to that of aspirin and histopathological sections showed a decrease in granulation tissue formation as compared to control. Interpretation & conclusions: Ranolazine demonstrated significant anti-inflammatory activity in acute and subacute models of inflammation and needs further evaluation for its use in reducing atherosclerosis.
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Lipases with their multifarious applications in a wide variety of fields are gaining the attention of industrial biotechnologists thus necessitating the need for screening new isolates with potential industrial applications. In the present study Taguchi DOE was employed to optimize the process parameters for lipase production by Emericella nidulans DAOM 222012, screened and isolated from Palm Oil Mill Effluent (POME) dump sites. This method allows the simultaneous study of several cultural factors while considering the mutual interaction among them and enhances the production within few experimental runs thereby greatly reducing the cost and time for process optimization. Four factors Viz., pH, carbon, nitrogen and surfactants each at 5 levels were considered and an orthogonal layout of L25 (54) was performed. The results indicated that Maltose (1%), Yeast extract (1%), Tween 80 (0.5%) and pH 6 are the significant factors for lipase production by Emericella nidulans DAOM 222012 using submerged fermentation studies.
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Xylanase (EC 3. 2. 1. 8), hydrolyzes xylo-oligosaccharides into D-xylose and required for complete hydrolysis of native cellulose and biomass conversion. It has broad range of applications in the pulp and paper, pharmaceutical and Agri-food industries. Fifty fungal species were isolated from the fouled soil around an oil refinery and screened for the production of xylanase enzyme by enrichment culture techniques. The isolated fungal strain was identified as Hypocrea lixii SS1 based on the results of biochemical tests and 18s rRNA sequencing. The phylogenetic tree was constructed using the MEGA 5 software. Further, Hypocrea lixii SS1 was tested for the ability to utilize the sunflower oil sludge (waste from the oil industry) as the sole carbon source for xylanase production. The growth characteristics of Hypocrea lixii SS1 were also studied and maximum growth was found on the 7th day of incubation. The fungus showed a remarkable xylanase production of 38.9 U/mL. Xylanase was purified using a combination of 0-50% NH4SO2 precipitation, DEAE-sepharose and Sephacryl S-200 chromatography. Single peak obtained in RP-HPLC confirms the purity of xylanase. Further the enzyme produced was affirmed as xylanase with its molecular weight (29 kDa) using SDS-PAGE.
Subject(s)
Soil Microbiology , Trichoderma/classification , Trichoderma/isolation & purification , Xylosidases/analysis , Chromatography, Liquid , Cluster Analysis , DNA, Fungal/chemistry , DNA, Fungal/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Electrophoresis, Polyacrylamide Gel , Molecular Sequence Data , Molecular Weight , Mycological Typing Techniques , Phylogeny , /genetics , Sequence Analysis, DNA , Trichoderma/enzymology , Trichoderma/growth & development , Xylosidases/chemistry , Xylosidases/isolation & purificationABSTRACT
This report describes analysis of paclitaxel, which is an antineoplastic drug used in the treatment of kaposi's sarcoma and cancer of the lung by isocratic high performace liquid chromatography with UV detection in pure form and rat plasma. The analysis was carried out using phenomenex C18 (250 x 4.6 mm, 5 μm particle size) column with a mobile phase consisting of acetonitrile and phosphate buffer (pH 5) in the ratio of 80:20%, v/v. Paclitaxel was eluted at the retention time of 5.3 min when operated at the flow rate of 1 ml/min and monitored by UV at 227 nm. Paclitaxel was extracted from rat plasma by simple LLE method using non- toxic ethyl acetate as extraction solvent. The linearity was accessed in the concentration range of 100-600 μg/mL with correlation coefficient of 0.9999 and percentage recovery of 99.86. The liquid chromatography method was extensively validated for linearity, accuracy, precision, LOD, LOQ and robustness. All these analytical validation parameters were observed to be satisfactory, which indicates the usefulness of method for determination of paclitaxel in pure form and rat plasma. No interfering peaks were observed during the analysis.
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The horseshoe kidney was originally regarded as a rare anatomical curiosity, but with the aid of retrograde pyelogram, intravenous urogram and renal arteriograms in this present age of diagnosis, the incidence of horseshoe kidney is estimated at 1 in 200-400 individuals or 1 in 700 autopsies and usually remains asymptomatic. The present report is concerned with a case of horseshoe kidney, which was observed during routine cadaveric dissection, for student education in anatomy dissection hall of Osmania medical college, in a male cadaver. The kidneys formed a U-shaped structure as a result of fusion at the inferior poles of the original kidneys by a parenchymatous isthmus. As a whole, the structure presented a typical horseshoe shape. The location of the kidney was lower than that of the normal kidney. The renal arterial system was almost normal except for a surplus artery into the isthmus that directly originated from the aorta, at the origin of inferior mesenteric artery. Venous drainage of both the kidneys and the isthmus was through two veins which opened independently into the inferior vena cava. The hila on both sides opened towards the ventral direction, and the ureters descended in front of the isthmus and entered the bladder normally. This report is being made because it affords material for a review of embryological and gross anatomy findings in a case of horseshoe kidney, which could help in a thorough urologic evaluation in diagnosed cases prior to any surgical intervention.
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Tamarindus indica Linn. fruits (Chincha) are extensively used in culinary preparations in Indian civilization. Its vast medicinal uses are documented in Ayurvedic classics and it can be used singly or as a component of various formulations. Besides fruit, the Kasta (wood) of T. indica L. is also important and used to prepare Kshara (alkaline extract) an Ayurvedic dosage form. Pharmacognostical and physicochemical details of Chincha Kasta are not available in authentic literature including API (Ayurvedic Pharmacopoeia of India). The study is an attempt in this direction. T. indica L. stem with heartwood was selected and morphological, microscopic and physicochemical standardization characters along with TLC fi nger print, and fl uorescence analysis were documented. Transverse section of stem showed important characters such as phelloderm, stone cells layer, fi ber groups, calcium oxalate, crystal fi bers, and tylosis in heartwood region. Four characteristic spots were observed under UV long wave, in thin layer chromatography with the solvent combination of toluene: ethyl acetate (8:2). The study can help correct identifi cation and standardization of this plant material.
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Withaniasomnifera [Ashwaganda] belonging to the family solanaceae is the subject of our present study. Withanoloides which are the major chemical constituents have been proved of interest because of their structural variations in the hybrids of different races. Docking is the process which brings the two structures together. In the present study we focus the extensive use of tool and graphical software for the identification of the binding energy of selected Withanolides like Withaferin -A, Withanolide-D from Withaniasomnifera and to screen the phytoconstituents that will dock/bind to the active sites of COX-2 enzyme. The relief from the symptoms of inflammation and pain can be by the Pharmacological inhibition of COX which involves the prediction of potential ligand for the treatment of inflammation. The energy value of docking between the target and the phytoconstituents under investigation and comparison with Diclofenac sodium was taken into consideration for coming into conclusion regarding the best pose and the binding ability
Subject(s)
Cyclooxygenase 2/chemistry , Cyclooxygenase 2 Inhibitors/chemistry , Binding Sites , Models, Molecular , Diclofenac/chemistryABSTRACT
Cytokinin independent-1 (CKI-1) gene was identified through its ability to confer cytokinin independent growth in Arabidopsis which has led to this gene being advocated as a selectable marker in plant transformation. Keeping this in view, CKI-1 gene was assessed as a selectable marker by transformation of tobacco (Nicotiana tabacum) and tomato (Lycopersicon esculentum L.). Overexpression of CKI-1 gene through Agrobacterium mediated transformation in tobacco and tomato conferred cytokinin independent shoot regeneration (in media devoid of cytokinin/plant growth regulators) in tobacco, but not in tomato wherein this ability (cytokinin independence) was conferred to T1 explants of CKI-1 transgenic tomato plant (T0) regenerated on cytokinin medium. Analysis of cytokinin levels revealed that cytokinin independent growth upon transformation with CKI-1 gene in tobacco (T0) and tomato (T1) was achieved through maintaining/regulating higher endogenous cytokinin levels and CKI-1 gene expression. Levels of CKI-1 transcripts assayed through quantitative RT-PCR suggested that there seemed to be a threshold level of endogenous cytokinin level, regulated due to external or internal supply via CKI-1 gene upto which CKI-1 gene expression correlated with endogenous cytokinin content and beyond that, either the gene expression was not induced or it remains same. With the incorporation of CKI-1 gene, it appeared that this threshold level of endogenous cytokinin might be reduced in crops like tomato to support shoot regeneration at lower concentration of cytokinin, but could not be made independent of external supply of cytokinin as in tobacco suggesting that use of CKI-1 gene as an effective alternate selection marker could not be universally applicable across the species. The results of the present study revealed that CKI-1 gene in addition to enhancing cytokinin levels, was also involved in contributing to the sensitivity to cytokinin and thus served as a positive regulator of cytokinin signal transduction.