ABSTRACT
Tuberculosis (TB) is an infectious disease, in which Mycobacterium tuberculosis is the causative agent. When a person progresses from infection to disease, they may not experience obvious symptoms for a long time, (e.g cough, fever, hemoptysis, weight loss etc). This might lead to delay in diagnosis and treatment seeking. One of the components of timely diagnosis and treatment of tuberculosis is estimating the delay in diagnosis of TB and assessing the factors contributing to the delay. To Aim and Objectives: describe the delays in the diagnosis and treatment of pulmonary Tuberculosis and to study the socio demographic determinants responsible for the same. A time bound institution based study was conducted in a tertiary care Methodology: hospital in department of pulmonary medicine. A total of 50 patients were included in the study by means of total enumeration. diagnosti Result: c and consultation delays were the most common cause of delays in seeking diagnosis and treatment of Pulmonary Tuberculosis.
ABSTRACT
Background: Different phenotypic detection method for theMethicillin Resistant Staphylococcus aureus is available.Objective: The aim of this present study was to evaluate theoxacillin disk diffusion test for the detection of methicillinresistant Staphylococcus aureus isolated from clinicalspecimens.Methodology: This cross-sectional study was planned to carryout in the Department of Microbiology and Immunology atBangabandhu Sheikh Mujib Medical University, Dhaka fromJanuary, 2010 to December, 2010 for a period of one (01)year. Different clinical specimens were collected from thepatients presented with infections at anybody sites. Isolationand identification of Staphylococcus aureus (S. aureus) wasperformed by staining, culture and biochemical tests. Oxacillindisk diffusion test was performed to detect the methicillinresistant Staphylococcus aureus. PCR was performed fordetection of the mecA gene for MRSA.Results: Out of the 22 suspected MRSA isolates 19 weremecA positive by PCR. The sensitivity and specificity ofoxacillin disc diffusion method for the detection of MRSA was84.2% (95 CI 60.4 to 96.6%) and 66.2% (95% CI 9.4 to99.2%) respectively. The positive predictive value and negativepredictive value of oxacillin disc diffusion method were 94.1%(95% CI 76.1 to 98.8%) and 40.0% (95% CI 15.2 to 71.2%)respectively. The accuracy was 81.8% (95% CI 66.7 to 95.3%).Conclusion: Oxacillin disk diffusion test shows high sensitivitywith moderate specificity for the detection of MRSA fromclinical specimens.